Western blotting of FOXA2 (A), HNF4α (B) and Actin (C) in CaCo-2 cells and human liver.

<p>Note, the HNF4α served as a positive control while Actin was used as loading control. Human liver nuclear extracts served as additional positive control. One of the siRNA oligonucleotides reduced FOXA2 protein significantly (see siRNA probe 3). This probe also affected expression of HNF4α while HNF6 plasmid expression had no effect on FOXA2 or HNF4α protein expression. M = molecular weight standard; LG = nuclear extracts of healthy liver; LM = nuclear extracts of colorectal liver metastases; PK = nuclear extracts of untreated Caco-2 cells; lanes 1-3 refers to different siRNA probes.