Effects of siRNA against raptor mRNA on K562 cells γ-globin gene expression.

<p>(A) Treatment of uninduced K562 cells with siRNA against raptor mRNA (black boxes) and siRNA against GAPDH mRNA (white boxes). (B) Effects of siRNA against raptor mRNA and siRNA against GAPDH mRNA on K562 cells induced to erythroid differentiation using a suboptimal concentration of MTH (15 nM). K562 cells were seeded at 10000 cell/100 μl following the Ambion transfection protocol using siPort NeoFX transfection reagent and 200 nM Silencer GAPDH siRNA and pre-designed siRNAs targeting raptor mRNA. Cells-to-cDNA II Kit was used to produce cDNA and RT-qPCR were conduced as reported in the methods section. The histograms represent the effects of si-GAPDH and si-raptor mRNA on GAPDH (white boxes), raptor (black boxes) and γ-globin (grey boxes) transcripts. The data represent the mean±S.D. from three independent experiments.