siRNA for Hes-1 inhibited proliferation of IEC-6 cells.

<p>Cells were plated onto 6-well plates. Inhibition of Jagged-2 with siRNA was carried out as mentioned above (see the Materials and Methods). <i>A</i>: After 48 h of culture, protein was extracted from IEC-6 cells. Western blot analysis showed that siRNA for Hes-1 downregulated protein expression of Hes-1 in IEC-6 cells compared with the si-NC or control group. GAPDH was used as loading control. <i>B</i>: Graphic representation of relative expression of Hes-1 normalized to GAPDH. Data are given as the means ± SDs (<i>n</i> = 5). **<i>p</i><0.01 versus control group. <i>C</i>: Cell count of IEC-6 cells was taken for control group, si-NC, and siRNA for Hes-1 group (magnification×400). <i>D</i>: IEC-6 cells were plated onto a 96-well plate. Inhibition of Hes-1 with siRNA was carried out as mentioned above (see the Materials and Methods). MTT assay showed that siRNA for Hes-1 decreased the cell number of IEC-6 cells significantly. Data are given as the means ± SDs (<i>n</i> = 10) **<i>p</i><0.01 versus control group. Control: IEC-6 cells were cultured normally. si-NC: IEC-6 cells were transfected with unrelated control siRNA. siRNA: IEC-6 cells were transfected with siRNA for Hes-1.