Phenotypic alterations of cells after siRNA mediated down-regulatin of OGDHL.

<p><b>A..... </b><b>MTT assay:</b> MTT assay 48 hours after transient transfection of OGDHL siRNA in HeLa and ME180 cell lines; Cell growth rate is expressed as absorbance at 570 to 650 nm <b>B..... </b><b>BrdU assay:</b> OGDHL siRNA increased cell proliferation of HeLa and ME180 cells as detected by DNA synthesis (*<i>P</i><0.001 and 0.001 for HeLa and ME180 cells respectively); <b>C..... </b><b>Caspase 3 assay:</b> Caspase-3 activities in HeLa and ME180 cells were measured in mock or 48 hours after OGDHL siRNA-transfected cells using the Caspase colorimetric assay kit. *<i>P</i><0.001. <b>D..... </b><b>Immunoblot analysis</b>: Immunoblotting analysis of Caspase 3 and PARP1/2 in HeLa and ME180 cell lines after siRNA mediated knockdown of OGDHL and <b>E..... </b><b>Invasive assay</b>: Invasive assay with HeLa cells 48 hours after transient down-regulation of OGDHL by siRNA and scramble siRNA (control). Representative photograph of HeLa cells that invaded the polycarbonate membrane of transwell chamber (Left). The number of HeLa cells that invaded the polycarbonate membrane of transwell chamber were significantly more when OGDHL expression were inhibited by siRNA (* <i>P</i><0.001) (right). The data represent the mean ±SD of three independent experiments, each done in triplicate.