The LNP 306O₁₃ facilitated dose dependent GAPDH mRNA silencing in Caco-2 monolayers 24 hours post-transfection.

<p>Caco-2 monolayer barrier function as measured by TEER was not affected by LNP mediated gene silencing. Dose response data (blue circles) indicate an EC₅₀ siGAPDH dose of 10 nM. TEER values (black squares) are reported 24 hours post-transfection relative to the time of transfection (t = 0), normalized to untreated cells. Error bars represent s.d. (n = 3–6).