Decreased cell proliferation in MIN6 cells overexpressing 11β-HSD1 in the presence of DHC.

<p>In A and B, MIN6-Vec and MIN6-HSD1 cells were cultured for 3 d with or without DHC (100 nM) and the 11β-HSD1 inhibitor (1 μM). Relative cell numbers were determined using the MTT assay. N = 10, **P<0.01 in untreated MIN6-HSD1 vs. MIN6-Vec cells using one-way ANOVA. @P<0.01 in DHC-treated vs. untreated MIN6-HSD1 cells using one-way ANOVA. In C and D, cells were cultured for 3 d with or without DHC and the 11β-HSD1 inhibitor. The relative rate of cell proliferation was determined using BrdU incorporation into newly synthesized DNA. N = 10, @P<0.01 in DHC-treated vs. untreated MIN6-HSD1 cells using one-way ANOVA.