αHS stimulates the proliferation of ECs and transformed cells in an HSPG-dependent manner.

<p><b>A</b>, HUVECs were grown in serum free medium in the absence (white bars) or the presence of AO4B08 (black bars; 1.25 µg/ml) and with the indicated concentration of heparin for 3 h and assessed for cell proliferation by the [³H]thymidine incorporation assay. *Statistically different from untreated control, P<0.05; # Statistically different from AO4B08-treated, P<0.05. Control cells were incubated without antibody. <b>B</b>, HUVECs were grown in serum free medium in the absence (white bars) or the presence of AO4B08 (black bars; 1.25 µg/ml) without (Ctrl) or with CS-6, CS4, or CS-0 (10 µg/ml), as indicated. Cell proliferation was determined by the [³H]thymidine incorporation assay. *Statistically different from untreated control, P<0.05. <b>C</b>, HUVECs were grown in serum free medium in the absence (white bars) or the presence of heparin (black bars; 10 µg/ml) without or with the different αHS antibody clones (1.25 µg/ml), as indicated. Cell proliferation was determined by the [³H]thymidine incorporation assay. *Statistically different from untreated control, P<0.05; # Statistically different from αHS-treated, P<0.05. <b>D</b>, Wild type (CHO-K1) cells and PG- deficient (PgsA-745) CHO cells were grown in serum free medium in the absence or the presence of different αHS antibody clones (1.25 µg/ml), as indicated. Cell proliferation was determined by the [³H]thymidine incorporation assay. *Statistically different from untreated control, P<0.05; # Statistically different from αHS-treated CHO-K1 cells, P<0.05. <b>E </b><b>and F</b>, Human umbilical artery ECs (HUAECs; E) and U-87 MG glioblastoma cells (F) were grown in serum free medium in the presence of AO4B08 (αHS) at the indicated concentrations and proliferation was determined by the [³H]thymidine incorporation assay. *Statistically different from untreated control, P<0.05.