A novel small molecule inhibitor of SCD activity reduces cell proliferation in human lung and breast cancer cells.

<p>A549 (A) and H1299 (B) cells were incubated with with different concentrations of CVT-11127 (CVT) or DMSO vehicle for 24 h, as described, and cell proliferation was determined by Crystal violet assay. For a similar analysis, H460 cells (C) were treated with 1 µm CVT-11127 for 24 h. For the determination of DNA synthesis, A549 (D) and H460 (E) cells were incubated with 10 µM and 5 µM CVT-11127 or vehicle for 24 h and pulsed with [³H]thymidine (1 µCi/dish) for 2 h at 37°C. Total [³H]-labeled DNA was precipitated, radioactivity was quantified in a scintillation counter and normalized to protein concentration. F, MCF-7 and MDA-MB-231 breast cancer cells, and WS-1 human skin fibroblasts were incubated with 10 µM CVT-11127 (CVT) or DMSO vehicle for 24 h and cell proliferation was assessed by crystal violet staining method. E, H460 cells were incubated for 48 h with 1 µM CVT in presence of 1, 10, 50 and 100 µM sodium oleate complexed with BSA (1∶2 BSA:fatty acid ratio). Cell incubated with DMSO vehicle were considered the control group. Cell growth was estimated by crystal violet staining method. Values represent the mean±S.D. of triplicate determinations. *, p<0.05 or less vs control, by Student's t test.