11β-HSD1 regulates proliferation of NHDFs.

<p>(a) The relative expression of 11β-HSD1 in NDHF assessed by rtPCR with 10% FBS or 1% FBS in culture media. GAPDH was used as an internal control. (b) 11β-HSD1 selective inhibitor was applied to NHDFs cultured in DMEM containing 2% FBS at indicated dose and proliferation of the cells was assessed by MTS assay 72 h later. DMSO was applied as vehicle control. The histograms indicate means and SDs for eight independent experiments. An asterisk (*) indicates a statistically significant difference from the vehicle treated group (P<0.05, Student's <i>t</i>-test). (c) The relative expressions of Col1a1, MMP-1, FGF-2, TGF-β treated with 11β-HSD1 selective inhibitor at indicated dose for 48 h assessed by rtPCR. GAPDH was used as an internal control.