Elevated proinflammatory cytokines in GR^CD11c-cre mice.

<p>(A–C, E, and F) Kinetics of LPS-induced cytokine levels in WT and GR^CD11c-cre mice. The sera used in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1002269#pbio.1002269.g001" target="_blank">Fig 1C</a> were also used to determine cytokine concentrations at each time point. Data shown are from four to eight animals per strain per time point pooled from multiple independent experiments. (D) Semiquantitative RT-PCR analysis of IFN-α4 transcription in DC subsets from WT and GR^CD11c-cre mice 1 hr after LPS challenge. β-actin was used as internal control. Results shown are one representative of two independent experiments with pooled splenocytes from two WT and two GR^CD11c-cre mice. (G) Splenocytes were prepared from mice 12 hr after LPS injection, and cultured in vitro for 4 hr with Brefeldin A. Intracellular IFN-γ was measured for gated NK and T cells by flow cytometry. One mouse from each genotype is shown out of two independent experiments, each using three mice per group. (H) Mean fluorescence intensity (MFI) and percentage of IFN-γ⁺ cells in WT and GR^CD11c-cre mice 12 hr post LPS challenge (<i>n</i> = 3). (I) GR^CD11c-cre or WT mice, each represented by an individual symbol, were injected with the indicated doses of neutralizing anti-IL-12 antibody 1 hr before challenge with a lethal dose of LPS (10 μg/g body weight). Surface body temperature at 30 hr is plotted. Results shown are pooled from two independent experiments, each with three to four mice per group. The data used to make this figure can be found in <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.1002269#pbio.1002269.s001" target="_blank">S1 Data</a>.