LPS-induced cytokine production by mouse PMφ is dependent on CCR8.

<p>(A) PMφ or (B) BMMφ collected from naïve WT or <i>CCR8^-/-</i> mice were stimulated with LPS (100 ng/mL) Pam3CSK4 (Pam, 100 ng/mL), zymosan (Zym, 1 μg/mL), PolyI:C (Pol, 100 μg/mL), flagellin (Fla, 1 μg/mL), or CpG-ODN (CpG, 1 μg/mL) for 24 h. The levels of cytokines in the culture medium were measured. (C) PMφ or BMMφ prepared from WT or CCR8^-/- mice was stimulated with LPS as above for 3 or 8 hours, and mRNA levels for IL-10 was measured with quantitative RT-PCR. Values were normalized using 18 s rRNA and shown as relative expression levels to WT cells without LPS of each time point. In (A–C), data are shown as mean plus a standard deviation (SD) determined from triplicate assays for each condition. *Statistically significant difference between WT and <i>CCR8^-/-</i> cells (unpaired two-tailed t test). (D) PMφ or BMMφ gated as F4/80⁺ cells were obtained from naïve WT mice and stained with anti-CCR8 antibody (solid line) or without antibody (shaded histogram) analyzed with flow cytometory.