GC suppresses in vivo IL-12 production by CD8⁺ DCs.

<p>(A) Six hr after LPS challenge, WT and GR^CD11c-cre splenocytes were analyzed for intracellular IL-12 levels. The right panels are histograms of CD8 staining of CD11c⁺ IL-12⁺ cells. (B) Intracellular IL-12 levels in CD8⁻ and CD8⁺ DCs between WT (shaded) and GR^CD11c-cre (solid line). One representative pair of a group of three is shown. (C) GC suppression of IL-12 production by CD8⁺ DCs ex vivo. Splenocytes were prepared from mice 3 hr after LPS injection and cultured with Brefeldin A in the presence or absence of corticosterone for 4 hr. Intracellular IL-12 levels on gated CD8⁺ DCs from one experiment are shown. The data are representative of three independent pairs of mice.