Altered production of IL-6 and/or IL-12 by myeloid DC from c1(88-100) and c1(70-100) mice following stimulation with TLR ligands.

<p>BMDC from 8-12 week-old mice were expanded with FLT3L and then cultured in the presence or absence of Imiquimod R837, Poly(I:C), CpG 2216, or LPS for 18h with GolgiStop (for IL-12) or GolgiPlug (for IL-6) being added for the last 6 h. The cells were then stained as outlined in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0075166#pone-0075166-g005" target="_blank">Figure 5</a> and the Methods. (A) Left panel shows representative dot plots indicating the regions used to gate B220 ⁺ CD11b^- pDC (top left box) and B220^-CD11b⁺ mDC (bottom right box) within the CD11c⁺ population. Shown to the right are representative histogram plots of IL-6 and IL-12 for B6 (solid grey) and c1(70-100) mice (black line) in unstimulated (Media) and stimulated (Poly(I:C) for IL-6 or CpG 2216 for IL-12) conditions. (B) Scatterplots showing the MFI for IL-6 and IL-12 expression on mDC. (C) IFN-α and IL-23 levels in the culture supernatants of BMDC as measured by ELISA. (D) MFI for TNF-α expression in mDC. Horizontal lines indicate the mean. Significance levels were determined by one-way ANOVA with Dunns’ post-test. The <i>p</i> values for significant differences between B6 and congenic mouse strains are shown with *p<0.05, **p<0.01. Bars with <i>p</i> values above denote significant differences between congenic strains.