SRG3 over-expression driven by the β-actin promoter reduced cytokine production in DCs following LPS stimulation.

<p>Flt3L-cultured BMDCs (A) from both WT and CD2-SRG3 Tg B6 mice and (B) from both WT and β-actin-SRG3 Tg B6 mice were stimulated with either vehicle or LPS (40, 200, or 1000 ng/ml) for 14 hrs, and subsequently, the percentage of the IL12p40-expressing population among the CD11c⁺ BMDCs was assessed via flow cytometry. Representative data from three independent experiments are shown (upper panel). The graph in lower panel represents mean percentage ± SD for the proportion of IL12p40 (n = 3; **P<0.01). (C) WT, β-actin-SRG3 Tg, and CD2-SRG3 Tg mice were i.p. injected with LPS (2 μg) or vehicle. Sixteen hrs later, intracellular TNFα, IL12p40, iNOS, and IL10 production was assessed in splenic CD11c⁺ DCs via flow cytometry. The means ± SD are shown in the graphs (n = 4; *P<0.05, **P<0.01).