The Log2FC values of randomly selected 17 genes (gray bar), including SNL-dependent 9 up- or 8 down-regulated genes, were compared to the results obtained from qRT-PCR (white bar). The Log2FC values were obtained by DEGs calculation between control and SNL groups. The values of qRT-PCR were calculated from the results of three independent experiments, and presented Means ± standard errors. (TIF)</p
<p>Data were analyzed by the 2<sup>–ΔΔCt</sup> method using GAPDH as a reference gene. The results a...
<p>Eight genes were selected, and their transcript levels were quantified by Q-RT-PCR. Results of Q-...
<p>(A) Expression pattern validation of transcriptomic data in SD13829 or BS02 by qRT-PCR. (B) Expre...
<p>Gene expression differences analyzed by qRT-PCR and RNA-seq are both exhibited. For qRT-PCR, erro...
<p>Results of qRT-PCR analysis of remarkable genes at Stage IV are shown as the relative expression ...
<p>Expression of eight genes, showing varied expression patterns in RNA-sequencing experiment, were ...
<p>(A) Expression ratios (Log<sub>2</sub><sup>FC</sup>) obtained by RNA-seq and qRT-PCR. The express...
<p>The black bars represent RNA-seq data, and the white bars represent the qRT-PCR data. The RNA-seq...
<p>(A) Close correlation (r<sup>2</sup> = 0.80) of 12 selected genes between two approaches. (B) Com...
<p>Twenty-two genes were arbitrary selected, from highly up-regulated to highly down-regulated conti...
<p>Red lines represent the RNA-seq results, while blue bars represent the qRT-PCR results. All qRT-P...
<p>The letter “A” and “B” in the RNA-Seq and qRT-PCR mean the comparison of expression profiles of s...
<p>A comparison of the results from RNA-seq and from qRT-PCR analyses of differentially expressed ge...
The grey-scale bars represent relative gene expression in control (dark grey) and treated plants (li...
Five genes were selected to verify RNA-Seq results using qRT-PCR. Graphs on the left (A, C, E, G, I,...
<p>Data were analyzed by the 2<sup>–ΔΔCt</sup> method using GAPDH as a reference gene. The results a...
<p>Eight genes were selected, and their transcript levels were quantified by Q-RT-PCR. Results of Q-...
<p>(A) Expression pattern validation of transcriptomic data in SD13829 or BS02 by qRT-PCR. (B) Expre...
<p>Gene expression differences analyzed by qRT-PCR and RNA-seq are both exhibited. For qRT-PCR, erro...
<p>Results of qRT-PCR analysis of remarkable genes at Stage IV are shown as the relative expression ...
<p>Expression of eight genes, showing varied expression patterns in RNA-sequencing experiment, were ...
<p>(A) Expression ratios (Log<sub>2</sub><sup>FC</sup>) obtained by RNA-seq and qRT-PCR. The express...
<p>The black bars represent RNA-seq data, and the white bars represent the qRT-PCR data. The RNA-seq...
<p>(A) Close correlation (r<sup>2</sup> = 0.80) of 12 selected genes between two approaches. (B) Com...
<p>Twenty-two genes were arbitrary selected, from highly up-regulated to highly down-regulated conti...
<p>Red lines represent the RNA-seq results, while blue bars represent the qRT-PCR results. All qRT-P...
<p>The letter “A” and “B” in the RNA-Seq and qRT-PCR mean the comparison of expression profiles of s...
<p>A comparison of the results from RNA-seq and from qRT-PCR analyses of differentially expressed ge...
The grey-scale bars represent relative gene expression in control (dark grey) and treated plants (li...
Five genes were selected to verify RNA-Seq results using qRT-PCR. Graphs on the left (A, C, E, G, I,...
<p>Data were analyzed by the 2<sup>–ΔΔCt</sup> method using GAPDH as a reference gene. The results a...
<p>Eight genes were selected, and their transcript levels were quantified by Q-RT-PCR. Results of Q-...
<p>(A) Expression pattern validation of transcriptomic data in SD13829 or BS02 by qRT-PCR. (B) Expre...