Confirmation of RNA-Seq data by quantitative RT-PCR (Q-RT-PCR).

<p>Eight genes were selected, and their transcript levels were quantified by Q-RT-PCR. Results of Q-RT-PCR are illustrated in parallel with the RNA-Seq data, and showed identical patterns. Data are averages from three independent experiments with three biological replicates, normalized by <i>TEF2</i> as an internal control, and presented as relative expression levels in comparison to the transcript levels in the cells cultured in the absence of <i>o</i>-vanillin. Bars represent standard deviations.