Validation of RNAseq expression analysis by qRT-PCR.

<p>Relative expression levels of genes determined by RNAseq to be differentially expressed <i>in vivo</i> compared to A. <i>in vitro</i> mid-log cultures and B. <i>in vitro</i> stationary phase cultures. Relative expression was assessed using the 2(-Delta Delta C(T)) method, error bars represent standard deviation of three independent biological replicates.