<p>Expression of eight genes, showing varied expression patterns in RNA-sequencing experiment, were analyzed in CTRL as well as SS seedlings using qRT-PCR. The error bars show standard error between two biological replicates used for this experiment.</p
<p>Data from qPCR are shown as means ± standard error (SE) of three replicates. RPKM from RNA-seq ar...
<p>Comparison of expression level of nine DEGs between RNA-Seq data and qRT-PCR assay. PCR primers a...
<p>The Y-axis represents the relative expression levels of the four samples. The unit for the RNA-se...
<p>Gene expression differences analyzed by qRT-PCR and RNA-seq are both exhibited. For qRT-PCR, erro...
The grey-scale bars represent relative gene expression in control (dark grey) and treated plants (li...
<p>Results of qPCR were presented in histograms, and results of RNA-Seq were listed in the embedded ...
<p>Data were analyzed by the 2<sup>–ΔΔCt</sup> method using GAPDH as a reference gene. The results a...
<p>Relative expression levels of genes determined by RNAseq to be differentially expressed <i>in viv...
Five genes were selected to verify RNA-Seq results using qRT-PCR. Graphs on the left (A, C, E, G, I,...
<p>In total, five genes including XIST (lincRNA) were selected for real time data validation. Relati...
<p>(A) Close correlation (r<sup>2</sup> = 0.80) of 12 selected genes between two approaches. (B) Com...
The Log2FC values of randomly selected 17 genes (gray bar), including SNL-dependent 9 up- or 8 down-...
<p>Red lines represent the RNA-seq results, while blue bars represent the qRT-PCR results. All qRT-P...
<p>White bars represent RNA-seq data, while the black bars represent mean values of log<sub>2</sub> ...
Relative expression levels of selected ten genes and 18S as an internal reference detected by RT-qPC...
<p>Data from qPCR are shown as means ± standard error (SE) of three replicates. RPKM from RNA-seq ar...
<p>Comparison of expression level of nine DEGs between RNA-Seq data and qRT-PCR assay. PCR primers a...
<p>The Y-axis represents the relative expression levels of the four samples. The unit for the RNA-se...
<p>Gene expression differences analyzed by qRT-PCR and RNA-seq are both exhibited. For qRT-PCR, erro...
The grey-scale bars represent relative gene expression in control (dark grey) and treated plants (li...
<p>Results of qPCR were presented in histograms, and results of RNA-Seq were listed in the embedded ...
<p>Data were analyzed by the 2<sup>–ΔΔCt</sup> method using GAPDH as a reference gene. The results a...
<p>Relative expression levels of genes determined by RNAseq to be differentially expressed <i>in viv...
Five genes were selected to verify RNA-Seq results using qRT-PCR. Graphs on the left (A, C, E, G, I,...
<p>In total, five genes including XIST (lincRNA) were selected for real time data validation. Relati...
<p>(A) Close correlation (r<sup>2</sup> = 0.80) of 12 selected genes between two approaches. (B) Com...
The Log2FC values of randomly selected 17 genes (gray bar), including SNL-dependent 9 up- or 8 down-...
<p>Red lines represent the RNA-seq results, while blue bars represent the qRT-PCR results. All qRT-P...
<p>White bars represent RNA-seq data, while the black bars represent mean values of log<sub>2</sub> ...
Relative expression levels of selected ten genes and 18S as an internal reference detected by RT-qPC...
<p>Data from qPCR are shown as means ± standard error (SE) of three replicates. RPKM from RNA-seq ar...
<p>Comparison of expression level of nine DEGs between RNA-Seq data and qRT-PCR assay. PCR primers a...
<p>The Y-axis represents the relative expression levels of the four samples. The unit for the RNA-se...
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