<i>RIM15</i> complement strain rescues <i>rim15Δ</i> ASR defects.

We restored RIM15 at the endogenous locus on the rim15Δ background and compared its ASR effect to that in the wild type and rim15Δ strains. Concentrations of H2O2 were calibrated to achieve a similar basal survival rates (open circles, Kruskal-Wallis rank sum test among the three groups P = 0.27). ASR-scores for the three strains are (95% CI and Wilcoxon signed-rank test P-values in the parenthesis): wild type 9.5 ([5.3, 16.9], P = 0.048); rim15::RIM15 11.6 ([8.6, 15.2], P = 0.048); rim15Δ 5.3 ([3.4, 7.5], P = 0.048). Based on Mann-Whitney U test, the ASR-score is not significantly different between either rim15Δ and wild type, or rim15::RIM15 and wild type (Holm-Bonferroni-corrected P-values = 0.6 and 0.3, respectively). rim15Δ has relatively weak effects on ASR (Fig 6D) and the wild type’s survival rates with primary stress has relatively large variance in this experiment. Both could result in the lack of statistical significance in the comparison between rim15Δ and wild type. Instead, the difference in ASR-score between rim15Δ and rim15::RIM15 has a P-value of 0.045. Combined, we conclude that rim15Δ reduces ASR and the complement strain rescues the defect. (PDF)