Effect of changes at HsdR Lys220 on the restriction phenotype of EcoR124I.

<p>^a Restriction activity was determined as the efficiency of plating of λvir.0 on the test strains relative to the efficiency of plating of λvir.0 on <i>E</i>. <i>coli</i> JM109(DE3) indicator (nonrestricting) strain as described in Methods. The values are the mean of at least three independent experiments. ^SD The standard deviation</p><p>^b Positive complementation was tested in r− host <i>E</i>. <i>coli</i> JM109(DE3)[pACMS] (r−m+).</p><p>^c Negative complementation was tested in r+ host <i>E</i>. <i>coli</i> JM109(DE3)[pKF650] (r+m+).</p><p>Effect of changes at HsdR Lys220 on the restriction phenotype of EcoR124I.