Type I interferon signaling is required for Ly6C expression on naïve CD8 T cells.

(A) Experimental design. Mice were infected with LCMV-Arm and 3 days later serum and naïve CD8 T cells were analyzed. (B) Amount of IFNα (pg/mL) within the serum of naïve and LCMV-Arm infected mice 3 days post-infection. (C) Frequency of Ly6C+ naïve CD8 T cells prior to and 3 days post-LCMV-Arm infection. (D) Ratio of Ly6C+:Ly6C- naïve CD8 T cells before and 3 days post-LCMV-Arm infection. (E) Experimental Design. WT or Ifnar-/- C57BL/6 mice were injected with PBS or 50 μg of poly(I:C). At various timepoints after injection, serum and naïve CD8 T cells were analyzed for IFNα and Ly6C expression, respectively. (F) Amount of IFNα in serum 2- and 168-hours post-PBS or poly(I:C) injection. (G) Frequency of Ly6C+ naïve CD8 T cells before and 1 day post-PBS or poly(I:C) injection. (H) Ratio of Ly6C+:Ly6C- naïve CD8 T cells before and 1-day post-injection. (I) Experimental design. Morbidity and mortality were monitored in mice that underwent cecal ligation and puncture (CLP) or sham (control) surgery. (J) Mortality and (K) morbidity of mice after surgery. (L) Ifnα message in splenocytes 3- and 6-hours post-surgery. Data are representative from at least three independent experiments with at least 5 mice per group. Error bars represent SEM. *p < 0.05, **p < 0.01, ***p < 0.001 ****p < 0.0001.