Naïve Ly6C⁺ CD8 T cells have increased accumulation early after viral challenge.

(A) Experimental design. Thy1.2+/1.2+ C57BL/6 mice received 5×103 Ly6C- (Thy1.1+/1.2+) and Ly6C+ (Thy1.1+/1.1+) naïve P14 CD8 T cells followed by an infection with LCMV-Arm, LCMV-Cl13, or VacV-GP33 1 day later via canonical infection routes. Cellular responses were monitored in peripheral blood 7- and 8-days post infections. (B) Flow plot example of injected cell master mix. Numbers indicate percentage of cells in each gate. P14 CD8 T cell responses following (C) LCMV-Arm, (D) LCMV-Cl13, or (E) VacV-GP33 infection. Top; Flow plot of transferred cells distinguished based on Thy1 disparity. Numbers are frequency of cells of all P14 CD8 T cells. Middle; number of transferred P14 CD8 T cells per mL of blood 7- and 8-days post infection. Bottom; Ratio of transferred P14 CD8 T cells that were Ly6C+ (Thy1.1+/1.1+) or Ly6C- (Thy1.1+/1.2+) at the time of co-transfer. Dotted line indicates input ratio of Ly6C+:Ly6C- naïve CD8 T cells. Data are representative from at least three independent experiments with at least 5 mice per group. Error bars represent SEM. *p < 0.05, **p < 0.01, ****p < 0.0001.