Ly6C⁺ naïve CD8 T cells have enhanced bystander responses following septic event.

(A) Frequency of Ly6C+ naïve CD8 T cells in spleen 28 days post-sham or CLP surgery. Frequency of IFNγ expressing Ly6C+ and Ly6C- naïve CD8 T cells 28 days post-surgery after ex vivo 5-hour stimulation with (B-C) IL-12/IL-18 or (D-E) IL-12/IL-18/IL-2. Cytokine production in naïve Ly6C+ and Ly6C- CD8 T cells from (F) Sham and (G) CLP-derived splenocytes following 5 hr ex vivo stimulation with PMA/Iono 28 days post-surgery. Left; IFNγ expression, middle; TNFα expression, right; IL2 expression. Data are representative from at least three independent experiments with at least 5 mice per group. Error bars represent SEM. *p < 0.05, **p < 0.01.