Co-localisation between NS5A, Core and LD.

Huh7.5 cells were electroporated with mJFH-1 WT and DI mutant C142A, C190A and E191A RNAs and seeded on to coverslips. At 72 hpe cells were stained with sheep anti-NS5A (white), rabbit anti-Core (green), BODIPY 558/568-C12 (red) and DAPI. Co-localisation was observed using Airyscan microscopy. Representative images are presented. A representative image of mock electroporated cells is shown in S6A Fig. Scale bars are 5 μm and 1 μm (insets).