Cellular localization of NA in infected MDCK-II cells without permeabilization. MDCK-II cells were infected with indicated viruses with an MOI of 1 for 8 (A) and 24 h (B). Cells were fixed and analyzed via confocal microscopy. Cell nuclei were counterstained with Hoechst33342. NA was detected by rabbit anti-N1 polyclonal antibodies. Confocal images were acquired with a Leica DMI6000 TCS SP5 confocal laser-scanning microscope (Leica Microsystems, Germany). Representative images of three independent experiments were processed using arivis vision4D (v3.4.0). Scale bar: 25 μm.</p
<p>Confocal microscopy images of Calu-3 cells are reported on (A) and in (B) for THP-1 macrophages. ...
<p>A549 cells were mock-infected or infected with 0.5 MOI rA2-His<sub>6</sub>-NS1 for 1 h. Cells wer...
<p>FG cells (A) and HINAE cells (B) were exposed to LCDV at 22°C for 2 h and stained with mouse anti...
<p>A549 cells were infected with the indicated viruses for 16 h at 34°C. Cells were processed for IF...
Detection of NA of indicated viruses with or without mutation in position 204 after infection of MDC...
<p>Vero cells were infected with the NSDVi isolate at a MOI of 1 TCID<sub>50</sub>. After 8, 12 or 1...
<p>R1 cells were induced with 0.25 µg/ml tetracycline and fixed at 2, 4, 8, 16 and 24 hours post-ind...
<p>Zanamivir-resistant mutation (Q136K) or oseltamivir-resistant mutation (H275Y) were introduced in...
<p>The anti-NP monoclonal antibody 3/1 and a rabbit antibody recognizing mCherry were used. Maximal ...
<p>(A) A549 cells were transfected with the plasmid pEGFP-Mex NS1/219 or pEGFP-Mex NS1/230. At 24 h ...
<p>(A) HeLa cells were grown on cover glass and transfected with pCAGGS encoding wild-type NP-NES3 o...
<p>Immunocitochemistry assays using anti-CypB rabbit polyclonal antibody and mouse monoclonal anti-N...
<p>SVEC4-10 cells were seeded in collagen-coated cover slips and incubated for three days to produce...
<p>HeNC2 cells were stimulated with Pam2CSK4 (100 ng/ml, panel A), Pam2CSK4 plus Hb (100 ng/ml and 5...
<p>SLK cells were infected with KSHV, fixed at the indicated time point, and analyzed for LANA and P...
<p>Confocal microscopy images of Calu-3 cells are reported on (A) and in (B) for THP-1 macrophages. ...
<p>A549 cells were mock-infected or infected with 0.5 MOI rA2-His<sub>6</sub>-NS1 for 1 h. Cells wer...
<p>FG cells (A) and HINAE cells (B) were exposed to LCDV at 22°C for 2 h and stained with mouse anti...
<p>A549 cells were infected with the indicated viruses for 16 h at 34°C. Cells were processed for IF...
Detection of NA of indicated viruses with or without mutation in position 204 after infection of MDC...
<p>Vero cells were infected with the NSDVi isolate at a MOI of 1 TCID<sub>50</sub>. After 8, 12 or 1...
<p>R1 cells were induced with 0.25 µg/ml tetracycline and fixed at 2, 4, 8, 16 and 24 hours post-ind...
<p>Zanamivir-resistant mutation (Q136K) or oseltamivir-resistant mutation (H275Y) were introduced in...
<p>The anti-NP monoclonal antibody 3/1 and a rabbit antibody recognizing mCherry were used. Maximal ...
<p>(A) A549 cells were transfected with the plasmid pEGFP-Mex NS1/219 or pEGFP-Mex NS1/230. At 24 h ...
<p>(A) HeLa cells were grown on cover glass and transfected with pCAGGS encoding wild-type NP-NES3 o...
<p>Immunocitochemistry assays using anti-CypB rabbit polyclonal antibody and mouse monoclonal anti-N...
<p>SVEC4-10 cells were seeded in collagen-coated cover slips and incubated for three days to produce...
<p>HeNC2 cells were stimulated with Pam2CSK4 (100 ng/ml, panel A), Pam2CSK4 plus Hb (100 ng/ml and 5...
<p>SLK cells were infected with KSHV, fixed at the indicated time point, and analyzed for LANA and P...
<p>Confocal microscopy images of Calu-3 cells are reported on (A) and in (B) for THP-1 macrophages. ...
<p>A549 cells were mock-infected or infected with 0.5 MOI rA2-His<sub>6</sub>-NS1 for 1 h. Cells wer...
<p>FG cells (A) and HINAE cells (B) were exposed to LCDV at 22°C for 2 h and stained with mouse anti...