NS5A dimer interface mutations impaired NS5A and core interaction.

<p><b>(</b>A) Confocal imaging analysis of cells at day 3 post-electroporation of HJ3-5/NS5A^YFP RNA with or without indicated individual mutations following core immunostaining (Alexa Fluor 405) and LipidTOX deep red neutral lipid stain. At the right is the enlarged area from individual channel or merged-images showing the core and NS5A localization relative to each other. (B) NS5A^YFP and core co-localization determined by Pearson correlation coefficient. (C) GFP immunoprecipitation (IP) followed by western blot analysis to detect NS5A and core interaction. Shown at the bottom is the relative NS5A and core co-immunoprecipitation (co-IP) efficiency from two independent experiments. (D) Core and LD co-localization determined by Pearson correlation coefficient.