Sub-cellular localization of 5-LO1 and 5-LO∆13 in resting and stimulated cells.

<p>HEK293 cells expressing FLAP which were transfected to express either 5-LO1 or 5-LO∆13, or which were transfected with a control vector (pcDNA) were stimulated with 1 μM A23187 and 10 μM AA or were incubated with their diluent (resting) for 10 minutes. Cells were then fixed and permeabilized and then incubated with rabbit anti-5-LO. Slides were then incubated with an Alexa488-conjugated secondary anti-rabbit antibody (green) and with DAPI (blue) to visualize nuclei, and slides were then mounted. Samples were analysed by confocal microscopy and images are presented on the left panels. The intensity of the signals could be visualized by intensity profiles (right panels) of the regions indicated by the white line on the merge images. Images are representative of three independent experiments. Scale bar represents 30 μm.