Intensity profiles of of 5-LO1 and 5-LO∆13 staining in resting and stimulated cells.

<p>HEK293 cells expressing FLAP which were transfected to express either 5-LO1 or 5-LO∆13 were stimulated with 1 μM A23187 and 10 μM AA or were incubated with their diluent (resting) for 10 minutes. Cells were then fixed and permeabilized and then incubated with rabbit anti-5-LO. Slides were then incubated with an Alexa488-conjugated secondary anti-rabbit antibody and with DAPI to visualize nuclei, and slides were then mounted. Samples were analysed by confocal microscopy and the intensity of the signals of cross-sections of cells were measured as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0132607#pone.0132607.g005" target="_blank">Fig 5</a>. <b>Top panels:</b> The intensity of each cross section was measured at the centre of the nucleus (N), at the nuclear envelope (E) identified by the edge of DAPI staining, at 1 μm and at 3 μm from the nuclear envelope (cytoplasmic side). Values are means ± SEM, n = 9. Values in the same figure without a common superscript are different, p<0.05. <b>Bottom panel:</b> Box and whisker plots showing the distance from the centre of the nucleus of the most intense anti-5-LO staining of cross sections. The box represents the two middle quartiles of data, the vertical line in the box is the median and the whiskers represent the minimum and maximum of all the data. *Median value different from others, p<0.05. Values in all panels are from intensity cross sections of 9 cells per condition from three different fields of view, each field representing a separate experiment.