Localisation of nanos1 mutant transcripts in stage VI oocytes.

<p>3′ and 5′ deletions used to create mutant transcripts are shown in schematic form (ORF, hatched; 5′ UTR, red; 3′ UTR, black). The map of the constructs is not drawn on a linear scale, but the residue numbers on the ruler correspond to the construct ends below. <i>mut-nanos1</i> consisted of the full length transcript with its start codon mutated to TTG. Oocytes were injected with Cy5 labelled mutant transcripts and examined after 48 h using constant gain settings on the confocal microscope. The subsequent localisation of the injected RNA was scored as plus or minus. Examples of the different degrees of localisation are shown below.