Distribution of <i>HeT-A</i> sense RNA in <i>wt</i> and mutant ovaries.

<p>Fluoresence in situ hybridization to <i>HeT-A</i> sense RNA (red). DNA is shown in blue. Images were obtained using 40x magnification, except those specified otherwise. Images for the three genotypes were captured with the same microscope settings. Images in (E-N) have been adjusted to a lower intensity in order to visualize the mRNA distribution. (A-D) <i>wt</i> ovaries. (A) Stage 5–6 egg chamber. Arrows indicate localization close to the nucleus of nurse cells. (B) Stage 9. Arrow shows localization inside the nucleus of nurse cells. (C) Stage 10. (D) Magnified view of the border cells boxed in (C). Arrow indicates localization in the nucleus of border cells. (E-H) <i>GIII</i> ovaries. (E) Stage 8. Arrow indicates intense localization in the nucleus of nurse cells. (F) Stage 10. (G) Magnification of the border cells boxed in (F). Arrow shows intense localization in the posterior side of border cells cytoplasm. (H) Stages 10a and 10b. This image was obtained with 20x magnification. (I-N) <i>aub</i> mutant ovaries. (I) Stage 4. Arrow indicates localization inside the oocyte. (J) Stages 6–7 (left) and 8 (right). Arrows show localization in the oocyte. (K) Stage 8. Arrows indicate localization in the oocyte and in the cytoplasm of nurse cells. (L) Stage 9. Arrows show localization in the oocyte and in the cytoplasm of nurse cells. (M) Stage 10. Arrow indicates localization at the anterior side of the oocyte. (N) Stage 10 nurse cells. Arrow shows detection in the cytoplasm of a nurse cell.