Localization of poly(A) RNA and RNA binding proteins in human oocyte.

<p>A) Single Z-stack from confocal images of human and mouse GV oocytes. RNA FISH detecting poly(A) RNA subpopulation (green) and DNA (blue). The gray scale shows separated light channels. The asterisk indicates cumulus cells. The experiment was repeated 3 times, with 15 mouse oocytes per experiment and 3 human oocytes per experiment. Scale bars 20 μm. B) Single Z-stack of human and mouse GV oocytes and 2-cell stage mouse embryos probed with 4E-BP1 (green) and eIF4A3 (red) and DAPI (blue). The gray scale shows separated channels. The experiment was repeated 3 times, with 15 mouse oocytes and 3 human oocytes. Scale bars 20 μm. The asterisk indicates cumulus cells.