Subcellular localization of 2xFYVE-GFP reporter in wildtype and VPS34/PI3K59F-RNAi oocytes.

Confocal fluorescent microscopy and DIC imaging of stage 10 egg chambers with oocyte-specific expression of 2xFYVE-GFP reporter from (A-C) wild type control or (D-F) oocyte co-expressing dsRNA again VPS34/PI3K59F, triple-labeled for GFP (green), Rab7 (red) and F-Actin (blue). Images are presented as overlaying in color or individual channels in gray, as annotated. Genotypes: (A-C). w*/ w1118; matalpha4-GAL-VP16 (BDSC #7062), UAS-GFP-myc-2xFYVE (BDSC #42712)/+; (D-F). w*/ w1118; matalpha4-GAL-VP16 (BDSC #7062), UAS-GFP-myc-2xFYVE (BDSC #42712)/+; P{TRiP.HMJ30324}attP40 (BDSC #64011)/+. The sizes of the scale bars as annotated. (TIF)