Variable knockdown efficiency by different RNAi lines in stage 10 oocytes.

Confocal fluorescent microscopy and DIC imaging of stage 10 egg chambers with oocyte-specific expression of dsRNA against (A, B) Rab5, (C, D) Rab4 and (E, F) Rab7, co-stained for endogenous Rab7 (green) and F-Actin (red), as annotated. (B, D, F) High-magnification view of the cortex regions highlighted in (A, C, E), respectively. Images are presented as overlaying images in color or as individual channels in gray, as annotated. Genotypes: The samples were from adult females flies heterozygous for matalpha4-GAL-VP16 (BDSC #7062) driver together with (A, B) P{TRiP.GL01872}attP40 (BL# 67877). (C, D) P{TRiP.HMS01100}attP2P (BDSC #33757). (E,F) P{y(+t7.7] v(+t1.8) = TRiP.JF02377}attP2 (BDSC#27051). The sizes of the scales as annotated inside images. (TIF)