The roles of Rab7 on yolk granule biogenesis in vitellogenic oocytes.

Confocal fluorescent microscopy and DIC imaging of stage 10 egg chambers with ectopic expression of (A-D) wildtype YFP-Rab7 or (E-H) constitutive active (CA) YFP-Rab7-CA in oocytes that are (A-F) co-labeled for anti-GFP (green), F-Actin and (A, B) anti-Rab7 or (C, D) lysotracker (red), or (G, H) by lysotracker (red) alone, shown as overlaying images of all the channels in color or in individual channels in gray, as annotated. (B, D, F) High-magnification view of the cortex regions highlighted in (A, C, E), respectively, as annotated. Genotypes: The samples were from adult female flies heterozygous for both matalpha4-GAL-VP16 driver (#7062) and (A-D) wildtype YFP-Rab7: y[1 w*; P{w(+mC) = UASp-YFP.Rab7}21/SM5 (#23641): (E, F) constitutive active YFP-Rab7-CA: P{UASp-YFP.Rab7.Q67L} (#24103).The sizes of the scale bars as annotated. (TIF)