Detection of in situ translation in oocyte and embryo.

<p>A) Proximity ligation assay (PLA) detects interaction of two translational components RPL24 and RPS6 in the oocyte and 2-cell embryo. Fluorescent foci indicate RPL24 and RPS6 interactions (green) in the oocyte and embryo. As negative controls, oocytes and 2-cell embryos were treated with the translational inhibitor puromycin, or a single RPS6 antibody was used. DNA stained with DAPI (blue); the gray scale shows separated channels. Scale bar 20μm. The experiment was repeated 3 times, with 25 oocytes/embryos per experiment. B) Graph shows quantification of RPL24 and RPS6 interactions in the whole cell volume. The values obtained from the oocyte were set as 1. The experiment was repeated 3 times, with 25 oocytes/embryos per experiment. Data are represented as mean ± s.d.; the value bars with <i>ns</i> are not significant, and the asterisk denotes statistically significant differences *p<0.05; **p<0.01; ***p<0.001.