Fig 6 -

Inactivation of Atp7b does not decrease DBH expression in mouse brain (a) Immunofluorescence staining for DBH shows enriched DBH positive cells (DBH+) in the locus coeruleus (LC) region in coronal sections of mice brain tissues. Scale bar, 20 μm. The (b) number and (c) area occupied by DBH+ cells in the left (LS) and right side (RS) of LC were compared between Atp7b-/- and WT (+/+) mice separately. The (d) total number of DBH+ cells and the (e) area occupied by the cells in LC region were compared between Atp7b-/- and WT mice. The data were analyzed using unpaired two tailed t-test. p ≤ 0.05 was considered statistically significant. (f) DBH protein (70 kDa) expressions were compared between Atp7b-/- and WT (+/+) mice brain using western blot. Expression of housekeeping gene GAPDH was considered as loading control. (g) Densitometric analysis of DBH bands compared to the corresponding GAPDH bands between Atp7b-/- and WT mice. (n = 4) The values represent the mean ± SD in triplicate. p ≤ 0.05 was considered statistically significant. Data analysis was performed using GraphPad Prism 9 software.