Cerebral DDAH activity is not increased in hDDAH-1 transgenic mice despite a significant difference in hDDAH-1 expression and translation.

<p>Muscular DDAH activity is increased but generally lower than cerebral DDAH activity. A Quantitative PCR. Relative expression in TG vs. WT mice. (n = 7–8) B Western blot, brain homogenate. Beta-tubulin and DDAH-1 antibodies (n = 3) C Cerebral and muscular DDAH activity. Brain and muscle homogenate (n = 6–8) D ADMA concentration. Brain homogenate (n = 6–8). Error bars represent standard deviation. WT wildtype; TG hDDAH-1 transgenic animals, n.s. not significant, ** p<0.01.