Me3H3K4 at the HD locus in R6/1 and R6/2 striatum and cerebellum.

<p>A. Schematic representation of the HD transgene expressed in R6 mice, showing location of primers used to amplify the region just downstream of the TSS in HD exon-1. B. ChIP analyses from the striatum and the cerebellum of R6/1 mice at 13- and 36-wk and from the striatum and cerebellum of R6/2 mice at 12-wk, using antibody to Me3H3K4. Error bars, sem; *, inter-tissue comparisons; §, inter-mouse line comparisons. *, p<0.05; §, p<0.05. C. Left panel. Global analysis of the level of Me3H3K4 at the HD locus. Me3H3K4 levels at the HD locus correspond to mean ChIP values at the HD promoter, HD exon-1 and HD intron-1. Error bars, sem; §, p<0.05. Right panel. Correlative analysis of CAG instability rates and Me3H3K4 levels at the HD locus. R6/1 mice of 13 and 36 weeks and R6/2 mice of 12 weeks were used for correlation analysis.