Distribution of D1 and D2 reporter proteins in acutely dissociated mouse striatal sections reveals an age-dependent increase in segregation.

<p>Characterization of tdTomato (D1, red) and GFP (D2, green) immunolabeling in neurons acutely dissociated from E18, P0 and P14 striatum from double-transgenic mice. <b>A</b>: Examples of acutely dissociated striatal neurons immunolabeled for tdTomato (D1, red) and GFP (D2, green). <b>B–D</b>: Summary diagrams presenting the results of quantifications performed from E18, P0 and P14 mice <b>(</b>tdTomato in B, GFP in C and both proteins in D). <b>E</b>: Single-cell RT-PCR from freshly dissociated P14 WT striatal cells. In this example (wells 2–14), nine collected neurons expressed D1 receptor mRNA and two collected neurons expressed D2L receptor mRNA. One neuron collected expressed both receptors mRNA (well 11). Positive control: whole mesencephalon RNA; negative control: water. <b>F</b>: Table summarizing the results of single-cell RT-PCR experiments performed with P0 and P14 WT mice.