Mosaic expression of the γ2 subunit in the brain of γ2 KD mice.

<p><b>A–F,</b> Representative images of sensorimotor cortex, hippocampal CA1 and cerebellum showing immunofluorescence labeling for the γ2 subunit. Note the reduced punctate labeling in γ2 KD brains as compared with WT. In γ2 KD cerebellum the co-existence of γ2-positive and γ2-negative PCs (asterisks) results in an uneven distribution of synaptic clusters in the molecular layer (ML). <b>G,</b> Quantification of γ2-positive puncta in brain regions of γ2 KD mice and WT littermates (***, p<0.001; unpaired <i>t</i>-test; n = 3 mice per group). OB, olfactory bulb (external plexiform layer); CX, sensorimotor cortex (layer V); CA1, CA1 (<i>stratum radiatum</i>); CB, cerebellum (molecular layer). <b>H,</b> Example traces of sIPSCs recorded from PCs in WT and γ2 KD mice. <b>I,</b> Quantitative analysis showing dramatically reduced amplitude of sIPSCs recorded from γ2 KD PCs (n = 17 cells) compared with WT (n = 11 cells; **, p = 0.0066; unpaired <i>t</i>-test). <b>J,</b> Reduced frequency of sIPSCs recorded from γ2 KD PCs (n = 17 cells) compared with WT (n = 11 cells; ***, p<0.0001; unpaired <i>t</i>-test) <b>K,</b> Normalized and superimposed representative traces of sIPSCs recorded from WT (gray) and KD cells (black). <b>L,</b> Quantification of current deactivation (τ_mean) in WT (n = 11) and KD cells (n = 17). <b>M,</b> Current onset kinetics (10–90% rise time) of sIPSCs from WT and γ2 KD neurons. <b>N,</b> Distributions of deactivation (τ_mean) and amplitude of sIPSCs recorded from PCs in WT and γ2 KD slices. Each data point represents the τ_mean and amplitude values of individual recordings. Arrows indicate the values of two PCs recorded from γ2 KD slices showing WT-like current properties. Data represent mean ± SEM (***, p<0.001; unpaired <i>t</i>-test). Scale bar: 25 µm.