Effect of TGFβ on IL1+OSM-induced <i>MMP13</i> expression.

SW1353 chondrocytes were stimulated with IL-1 (0.5 ng/ml) + OSM (10 ng/ml) ± TGFβ (10 ng/ml) for the indicated durations. qPCR was then performed on isolated mRNA to measure MMP13 expression whilst glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used for normalisation purposes. (A) Data are presented as fold change relative to IL-1+OSM (normalized to 1.0 for each time point; mean ± SEM). (B) Data are presented as fold change relative to control. Data are pooled from at least 4 independent experiments (n ≥ 22) and statistical comparisons performed using an unpaired Student’s t-test, where ***p S1 File.