The effects of Anti-TGF treatment, on MMP-13 mRNA expression.

Deterministic simulation results for the complete model, with an extra reaction to allow RUNX2 inactivation. The data show the change in MMP-13 mRNA across a 30-month simulation time. IL-1+OSM were triggered using events at 4, 9 and 23 months. The model also contained the species “Anti-TGF” which targeted and degraded both active and inactive TGFβ. Anti-TGF concentration was at zero when the simulation started. A) At 6 months Anti-TGF was added to the system, using an event. Anti-TGF was added either once, twice or thrice with a 24 days interval between each addition. B) At 6 months Anti-TGF was added to the system, using an event. Anti-TGF was added three times to the model with either a 1-day, 6-day, 12-day or 24-day interval between each addition. C) At 15 months Anti-TGF was added to the system, using an event. Anti-TGF was added either once, twice or thrice with a 24 day interval between each addition. D) At 19 months Anti-TGF was added to the system, using an event. Anti-TGF was added three times to the model with either a 1-day, 6-day, 12-day or 24-day interval between each addition.