TGF-β expression in normal and osteoarthritic chondrocytes and effect of TGF-β treatment on SREBP-2, HMGCR, phospho-PI3K and MMP-13 expression.

<p>(<b>A</b>) TGF-β expression levels in normal and osteoarthritic chondrocytes detected by ELISA. *p<0.05 (<b>B</b>) Western blot showing SREBP-2, HMGCR and phospho-PI3K protein expression levels in normal and osteoarthritic chondrocytes treated with 10 ng/ml TGF-β for 0.5, 2, 6, and 24 h. β-actin was used as loading control. The same membrane was reprobed without stripping. (<b>C</b>) SREBP-2, HMGCR and MMP-13 mRNA in cultured normal chondrocytes following treatment with 10 ng/ml TGF-β for 0.5, 2, 6 and 24 h. All experiments were performed in duplicate and data are expressed as mean. *p<0.05 (<b>D</b>) MMP-13 protein expression levels in normal chondrocytes following treatment with 10 ng/ml TGF-β for 0.5, 2, 6 and 24 h detected by ELISA. *p<0.05.