MOESM3 of High-efficiency expression and secretion of human FGF21 in Bacillus subtilis by intercalation of a mini-cistron cassette and combinatorial optimization of cell regulatory components

Additional file 2 of Optimization of expression, purification and secretion of functional recombinant human growth hormone in Escherichia coli using modified staphylococcal protein a signal peptide

Garshasb Rigi (11283161)
Amin Rostami (810255)
Habib Ghomi (11283164)
Gholamreza Ahmadian (3410240)
Vasiqe Sadat Mirbagheri (11283167)
Meisam Jeiranikhameneh (3803341)
Majid Vahed (5003837)
Sahel Rahimi (11283170)

August 2021

Additional file 2. The original, unprocessed and uncropped version of the Fig. 7 in manuscript

MOESM1 of Bioprospecting of novel thermostable β-glucosidase from Bacillus subtilis RA10 and its application in biomass hydrolysis

Rameshwar Tiwari (4107112)
Puneet Singh (3930716)
Surender Singh (152945)
Pawan Nain (4544962)
Lata Nain (682732)
Pratyoosh Shukla (3930719)

October 2017

Additional file 1: Figure S1. Qualitative screening of β-glucosidase producing microbes on agar medi...

Additional file 3: of A nutrient-dependent division antagonist is regulated post-translationally by the Clp proteases in Bacillus subtilis

Norbert Hill (5072483)
Jason Zuke (5072477)
P. Buske (5072480)
An-Chun Chien (5072474)
Petra Levin (3558365)

April 2018

Figure S3. UgtP oligomer mutant has WT localization and delays cell division in a ÎpgcA background;...

MOESM2 of High-efficiency expression and secretion of human FGF21 in Bacillus subtilis by intercalation of a mini-cistron cassette and combinatorial optimization of cell regulatory components

Dandan Li (391591)
Gang Fu (728533)
Ran Tu (4349167)
Zhaoxia Jin (1500538)
Dawei Zhang (58801)

February 2019

Additional file 2: Figure S2. SDS-PAGE analysis of the purified secreted rhFGF21 with C-terminal His...

MOESM5 of High-efficiency expression and secretion of human FGF21 in Bacillus subtilis by intercalation of a mini-cistron cassette and combinatorial optimization of cell regulatory components

Dandan Li (391591)
Gang Fu (728533)
Ran Tu (4349167)
Zhaoxia Jin (1500538)
Dawei Zhang (58801)

February 2019

Additional file 5: Table S1. Summary of in vitro activities of rhFGF21 from Kno6cf and hFGF21 standa...

MOESM1 of High-efficiency expression and secretion of human FGF21 in Bacillus subtilis by intercalation of a mini-cistron cassette and combinatorial optimization of cell regulatory components

Dandan Li (391591)
Gang Fu (728533)
Ran Tu (4349167)
Zhaoxia Jin (1500538)
Dawei Zhang (58801)

February 2019

Additional file 1: Figure S1. The relative expression level of rhFGF21 from different B. subtilis st...

MOESM4 of High-efficiency expression and secretion of human FGF21 in Bacillus subtilis by intercalation of a mini-cistron cassette and combinatorial optimization of cell regulatory components

Dandan Li (391591)
Gang Fu (728533)
Ran Tu (4349167)
Zhaoxia Jin (1500538)
Dawei Zhang (58801)

February 2019

Additional file 4: Figure S4. ERK1/2 phosphorylation assay of hFGF21 standard and rhFGF21 from Kno6c...

High-efficiency expression and secretion of human FGF21 in Bacillus subtilis by intercalation of a mini-cistron cassette and combinatorial optimization of cell regulatory components

Dandan Li
Gang Fu
Ran Tu
Zhaoxia Jin
Dawei Zhang

January 2019

Abstract Background Recombinant human Fibroblast growth factor 21 (rhFGF21) is an endocrine hormone ...

Preparation of cFGF-21.

Pengfei Xu (301785)
Yingjie Zhang (1234773)
Xinghao Jiang (2802682)
Junyan Li (238758)
Liying Song (2802685)
Mir Hasson Khoso (2802694)
Yunye Liu (2802691)
Qiang Wu (31071)
Guiping Ren (413780)
Deshan Li (2802688)

May 2016

<p>(A) SDS-PAGE gel analysis of cFGF-21 expression. Lane 1: Protein molecular mass marker; lane 2: T...

Construction and expression analyses of FGF15 and FGF19 Plasmids.

Bo Kong (172557)
Grace L. Guo (172561)

January 2014

<p>A): Schematic maps of plasmids. Truncated FGF15 or FGF19 gene (without signal peptide) was locate...

Additional file 3 of Optimization of expression, purification and secretion of functional recombinant human growth hormone in Escherichia coli using modified staphylococcal protein a signal peptide

Garshasb Rigi (11283161)
Amin Rostami (810255)
Habib Ghomi (11283164)
Gholamreza Ahmadian (3410240)
Vasiqe Sadat Mirbagheri (11283167)
Meisam Jeiranikhameneh (3803341)
Majid Vahed (5003837)
Sahel Rahimi (11283170)

August 2021

Additional file 3. The original, unprocessed and uncropped version of the Fig. 8 in manuscript

Additional file 3 of FGF12 is a novel component of the nucleolar NOLC1/TCOF1 ribosome biogenesis complex

Martyna Sochacka (14149008)
Radoslaw Karelus (14149011)
Lukasz Opalinski (14149014)
Daniel Krowarsch (14149017)
Martyna Biadun (14149020)
Jacek Otlewski (14149023)
Malgorzata Zakrzewska (14149026)

November 2022

Additional file 2: Fig. S1. Figure S1. Cellular localization of FHF proteins. A Western blotting ana...

MOESM7 of Enhancing surfactin production by using systematic CRISPRi repression to screen amino acid biosynthesis genes in Bacillus subtilis

Congya Wang (6731342)
Yingxiu Cao (1489501)
Yongping Wang (66268)
Liming Sun (1589266)
Hao Song (383912)

May 2019

Additional file 7: Figure S5. HPLC analysis of surfactin. Peak-1 contains a C13-β-hydroxy fatty acid...

MOESM1 of E. coli HMS174(DE3) is a sustainable alternative to BL21(DE3)

Johanna Hausjell (5913437)
Julia Weissensteiner (4357780)
Christian Molitor (660023)
Heidi Halbwirth (411270)
Oliver Spadiut (104825)

October 2018

Additional file 1: Figure S1. FHT production by HMS174(DE3) during the characterization-cultivation ...

MOESM3 of Module and individual domain deletions of NRPS to produce plipastatin derivatives in Bacillus subtilis

Ling Gao (429702)
Jianping Guo (299686)
Yun Fan (207346)
Zhi Ma (244851)
Zhaoxin Lu (701734)
Chong Zhang (418677)
Haizhen Zhao (3351623)
Xiaomei Bie (739007)

May 2018

Additional file 3: Figure S1. ESI–MS/MS spectra of protonated ions [M + H]+ at m/z 1313.7268 (A), m/...

Additional file 2 of Optimization of expression, purification and secretion of functional recombinant human growth hormone in Escherichia coli using modified staphylococcal protein a signal peptide

Garshasb Rigi (11283161)
Amin Rostami (810255)
Habib Ghomi (11283164)
Gholamreza Ahmadian (3410240)
Vasiqe Sadat Mirbagheri (11283167)
Meisam Jeiranikhameneh (3803341)
Majid Vahed (5003837)
Sahel Rahimi (11283170)

August 2021

Additional file 2. The original, unprocessed and uncropped version of the Fig. 7 in manuscript

MOESM1 of Bioprospecting of novel thermostable β-glucosidase from Bacillus subtilis RA10 and its application in biomass hydrolysis

Rameshwar Tiwari (4107112)
Puneet Singh (3930716)
Surender Singh (152945)
Pawan Nain (4544962)
Lata Nain (682732)
Pratyoosh Shukla (3930719)

October 2017

Additional file 1: Figure S1. Qualitative screening of β-glucosidase producing microbes on agar medi...

Additional file 3: of A nutrient-dependent division antagonist is regulated post-translationally by the Clp proteases in Bacillus subtilis

Norbert Hill (5072483)
Jason Zuke (5072477)
P. Buske (5072480)
An-Chun Chien (5072474)
Petra Levin (3558365)

April 2018

Figure S3. UgtP oligomer mutant has WT localization and delays cell division in a ÎpgcA background;...

MOESM2 of High-efficiency expression and secretion of human FGF21 in Bacillus subtilis by intercalation of a mini-cistron cassette and combinatorial optimization of cell regulatory components

Dandan Li (391591)
Gang Fu (728533)
Ran Tu (4349167)
Zhaoxia Jin (1500538)
Dawei Zhang (58801)

February 2019

Additional file 2: Figure S2. SDS-PAGE analysis of the purified secreted rhFGF21 with C-terminal His...

MOESM5 of High-efficiency expression and secretion of human FGF21 in Bacillus subtilis by intercalation of a mini-cistron cassette and combinatorial optimization of cell regulatory components

Dandan Li (391591)
Gang Fu (728533)
Ran Tu (4349167)
Zhaoxia Jin (1500538)
Dawei Zhang (58801)

February 2019

Additional file 5: Table S1. Summary of in vitro activities of rhFGF21 from Kno6cf and hFGF21 standa...

MOESM1 of High-efficiency expression and secretion of human FGF21 in Bacillus subtilis by intercalation of a mini-cistron cassette and combinatorial optimization of cell regulatory components

Dandan Li (391591)
Gang Fu (728533)
Ran Tu (4349167)
Zhaoxia Jin (1500538)
Dawei Zhang (58801)

February 2019

Additional file 1: Figure S1. The relative expression level of rhFGF21 from different B. subtilis st...

MOESM4 of High-efficiency expression and secretion of human FGF21 in Bacillus subtilis by intercalation of a mini-cistron cassette and combinatorial optimization of cell regulatory components

Dandan Li (391591)
Gang Fu (728533)
Ran Tu (4349167)
Zhaoxia Jin (1500538)
Dawei Zhang (58801)

February 2019

Additional file 4: Figure S4. ERK1/2 phosphorylation assay of hFGF21 standard and rhFGF21 from Kno6c...

High-efficiency expression and secretion of human FGF21 in Bacillus subtilis by intercalation of a mini-cistron cassette and combinatorial optimization of cell regulatory components

Dandan Li
Gang Fu
Ran Tu
Zhaoxia Jin
Dawei Zhang

January 2019

Abstract Background Recombinant human Fibroblast growth factor 21 (rhFGF21) is an endocrine hormone ...

Preparation of cFGF-21.

Pengfei Xu (301785)
Yingjie Zhang (1234773)
Xinghao Jiang (2802682)
Junyan Li (238758)
Liying Song (2802685)
Mir Hasson Khoso (2802694)
Yunye Liu (2802691)
Qiang Wu (31071)
Guiping Ren (413780)
Deshan Li (2802688)

May 2016

<p>(A) SDS-PAGE gel analysis of cFGF-21 expression. Lane 1: Protein molecular mass marker; lane 2: T...

Construction and expression analyses of FGF15 and FGF19 Plasmids.

Bo Kong (172557)
Grace L. Guo (172561)

January 2014

<p>A): Schematic maps of plasmids. Truncated FGF15 or FGF19 gene (without signal peptide) was locate...

Additional file 3 of Optimization of expression, purification and secretion of functional recombinant human growth hormone in Escherichia coli using modified staphylococcal protein a signal peptide

Garshasb Rigi (11283161)
Amin Rostami (810255)
Habib Ghomi (11283164)
Gholamreza Ahmadian (3410240)
Vasiqe Sadat Mirbagheri (11283167)
Meisam Jeiranikhameneh (3803341)
Majid Vahed (5003837)
Sahel Rahimi (11283170)

August 2021

Additional file 3. The original, unprocessed and uncropped version of the Fig. 8 in manuscript

Additional file 3 of FGF12 is a novel component of the nucleolar NOLC1/TCOF1 ribosome biogenesis complex

Martyna Sochacka (14149008)
Radoslaw Karelus (14149011)
Lukasz Opalinski (14149014)
Daniel Krowarsch (14149017)
Martyna Biadun (14149020)
Jacek Otlewski (14149023)
Malgorzata Zakrzewska (14149026)

November 2022

Additional file 2: Fig. S1. Figure S1. Cellular localization of FHF proteins. A Western blotting ana...

MOESM7 of Enhancing surfactin production by using systematic CRISPRi repression to screen amino acid biosynthesis genes in Bacillus subtilis

Congya Wang (6731342)
Yingxiu Cao (1489501)
Yongping Wang (66268)
Liming Sun (1589266)
Hao Song (383912)

May 2019

Additional file 7: Figure S5. HPLC analysis of surfactin. Peak-1 contains a C13-β-hydroxy fatty acid...

MOESM1 of E. coli HMS174(DE3) is a sustainable alternative to BL21(DE3)

Johanna Hausjell (5913437)
Julia Weissensteiner (4357780)
Christian Molitor (660023)
Heidi Halbwirth (411270)
Oliver Spadiut (104825)

October 2018

Additional file 1: Figure S1. FHT production by HMS174(DE3) during the characterization-cultivation ...

MOESM3 of Module and individual domain deletions of NRPS to produce plipastatin derivatives in Bacillus subtilis

Ling Gao (429702)
Jianping Guo (299686)
Yun Fan (207346)
Zhi Ma (244851)
Zhaoxin Lu (701734)
Chong Zhang (418677)
Haizhen Zhao (3351623)
Xiaomei Bie (739007)

May 2018

Additional file 3: Figure S1. ESI–MS/MS spectra of protonated ions [M + H]+ at m/z 1313.7268 (A), m/...

Additional file 2 of Optimization of expression, purification and secretion of functional recombinant human growth hormone in Escherichia coli using modified staphylococcal protein a signal peptide

Garshasb Rigi (11283161)
Amin Rostami (810255)
Habib Ghomi (11283164)
Gholamreza Ahmadian (3410240)
Vasiqe Sadat Mirbagheri (11283167)
Meisam Jeiranikhameneh (3803341)
Majid Vahed (5003837)
Sahel Rahimi (11283170)

August 2021

Additional file 2. The original, unprocessed and uncropped version of the Fig. 7 in manuscript

MOESM1 of Bioprospecting of novel thermostable β-glucosidase from Bacillus subtilis RA10 and its application in biomass hydrolysis

Rameshwar Tiwari (4107112)
Puneet Singh (3930716)
Surender Singh (152945)
Pawan Nain (4544962)
Lata Nain (682732)
Pratyoosh Shukla (3930719)

October 2017

Additional file 1: Figure S1. Qualitative screening of β-glucosidase producing microbes on agar medi...

Additional file 3: of A nutrient-dependent division antagonist is regulated post-translationally by the Clp proteases in Bacillus subtilis

Norbert Hill (5072483)
Jason Zuke (5072477)
P. Buske (5072480)
An-Chun Chien (5072474)
Petra Levin (3558365)

April 2018

Figure S3. UgtP oligomer mutant has WT localization and delays cell division in a ÎpgcA background;...

MOESM3 of High-efficiency expression and secretion of human FGF21 in Bacillus subtilis by intercalation of a mini-cistron cassette and combinatorial optimization of cell regulatory components

Abstract

Extracted data

MOESM3 of High-efficiency expression and secretion of human FGF21 in Bacillus subtilis by intercalation of a mini-cistron cassette and combinatorial optimization of cell regulatory components

Abstract

Extracted data

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