MOESM5 of High-efficiency expression and secretion of human FGF21 in Bacillus subtilis by intercalation of a mini-cistron cassette and combinatorial optimization of cell regulatory components

MOESM5 of A particular silent codon exchange in a recombinant gene greatly influences host cell metabolic activity

Natalie Rahmen (3496205)
Christian Schlupp (3496196)
Hitoshi Mitsunaga (3496214)
Alexander Fulton (2579620)
Tita Aryani (3496211)
Lara Esch (3496199)
Ulrich Schaffrath (3496208)
Eiichiro Fukuzaki (3496202)
Karl-Erich Jaeger (125594)
Jochen Büchs (3437060)

October 2015

Additional file 5. Product formation and sum of relative transcript abundances of four E. coli BL21(...

Preparation of cFGF-21.

Pengfei Xu (301785)
Yingjie Zhang (1234773)
Xinghao Jiang (2802682)
Junyan Li (238758)
Liying Song (2802685)
Mir Hasson Khoso (2802694)
Yunye Liu (2802691)
Qiang Wu (31071)
Guiping Ren (413780)
Deshan Li (2802688)

May 2016

(A) SDS-PAGE gel analysis of cFGF-21 expression. Lane 1: Protein molecular mass marker; lane 2: T...

Human FGF-21 is digested by FAP but not PREP.

Andrew L. Coppage (2560108)
Kathryn R. Heard (2560111)
Matthew T. DiMare (2560105)
Yuxin Liu (505575)
Wengen Wu (1883827)
Jack H. Lai (1883839)
William W. Bachovchin (1883854)

March 2016

(A) Human FGF-21 is cleaved by FAP. Recombinant human FGF-21 was digested by recombinant human FA...

MOESM1 of High-efficiency expression and secretion of human FGF21 in Bacillus subtilis by intercalation of a mini-cistron cassette and combinatorial optimization of cell regulatory components

Dandan Li (391591)
Gang Fu (728533)
Ran Tu (4349167)
Zhaoxia Jin (1500538)
Dawei Zhang (58801)

February 2019

Additional file 1: Figure S1. The relative expression level of rhFGF21 from different B. subtilis st...

MOESM4 of High-efficiency expression and secretion of human FGF21 in Bacillus subtilis by intercalation of a mini-cistron cassette and combinatorial optimization of cell regulatory components

Dandan Li (391591)
Gang Fu (728533)
Ran Tu (4349167)
Zhaoxia Jin (1500538)
Dawei Zhang (58801)

February 2019

Additional file 4: Figure S4. ERK1/2 phosphorylation assay of hFGF21 standard and rhFGF21 from Kno6c...

MOESM3 of High-efficiency expression and secretion of human FGF21 in Bacillus subtilis by intercalation of a mini-cistron cassette and combinatorial optimization of cell regulatory components

Dandan Li (391591)
Gang Fu (728533)
Ran Tu (4349167)
Zhaoxia Jin (1500538)
Dawei Zhang (58801)

February 2019

Additional file 3: Figure S3. LCâMS analysis of rhFGF21 protein expressed and purified from B. sub...

MOESM2 of High-efficiency expression and secretion of human FGF21 in Bacillus subtilis by intercalation of a mini-cistron cassette and combinatorial optimization of cell regulatory components

Dandan Li (391591)
Gang Fu (728533)
Ran Tu (4349167)
Zhaoxia Jin (1500538)
Dawei Zhang (58801)

February 2019

Additional file 2: Figure S2. SDS-PAGE analysis of the purified secreted rhFGF21 with C-terminal His...

High-efficiency expression and secretion of human FGF21 in Bacillus subtilis by intercalation of a mini-cistron cassette and combinatorial optimization of cell regulatory components

Dandan Li
Gang Fu
Ran Tu
Zhaoxia Jin
Dawei Zhang

January 2019

Abstract Background Recombinant human Fibroblast growth factor 21 (rhFGF21) is an endocrine hormone ...

Cloning, expression and purification of human fibroblast growth factor 21 in Escherichia coli and Pichia pastoris / Farnaz Emamdoust

Farnaz , Emamdoust

May 2018

Fibroblast Growth Factor 21 (FGF21) is a novel target with potential anti diabetic properties that ...

MOESM1 of Combinatorial pathway enzyme engineering and host engineering overcomes pyruvate overflow and enhances overproduction of N-acetylglucosamine in Bacillus subtilis

Wenlong Ma (6175187)
Yanfeng Liu (405866)
Xueqin Lv (279878)
Jianghua Li (390525)
Guocheng Du (390526)
Long Liu (390523)

January 2019

Additional file 1: Table S1. Primers used in this study. Fig S1. The mutagenesis selection process. ...

In vitro activity of Fc-FGF21(RG) relative to native FGF21 and Fc-FGF21 wild type.

Randy Hecht (117873)
Yue-Sheng Li (117877)
Jeonghoon Sun (117881)
Ed Belouski (117884)
Michael Hall (117887)
Todd Hager (117889)
Junming Yie (117890)
Wei Wang (17594)
Dwight Winters (117892)
Stephen Smith (117894)
Chris Spahr (117896)
Lei-Ting Tam (117897)
Zhongnan Shen (117899)
Shanaka Stanislaus (117901)
Narumol Chinookoswong (117903)
Yvonne Lau (117907)
Allen Sickmier (117911)
Mark Leo Michaels (117917)
Thomas Boone (117922)
Murielle M. Véniant (117927)
Jing Xu (15337)

November 2012

(A) Structural diagram of Fc-FGF21(RG), an E coli-expressed homodimeric Fc-FGF21 fusion protein c...

Table1_Scaling up production of recombinant human basic fibroblast growth factor in an Escherichia coli BL21(DE3) plysS strain and evaluation of its pro-wound healing efficacy.DOCX

Le Li (356426)
Bingjie Yu (694763)
Yingji Lai (17902871)
Siyuan Shen (649230)
Yawei Yan (8399274)
Guojun Dong (389687)
Xiangyun Gao (400310)
Yanrong Cao (10164943)
Caojie Ge (17902874)
Liqin Zhu (8982803)
Huan Liu (281351)
Shanhui Tao (17902877)
Zhiang Yao (17902880)
Shijun Li (165293)
Xiaojie Wang (166442)
Qi Hui (783264)

February 2024

Introduction: Human basic fibroblast growth factor (hbFGF) is a highly valuable multifunctional prot...

DataSheet5_Scaling up production of recombinant human basic fibroblast growth factor in an Escherichia coli BL21(DE3) plysS strain and evaluation of its pro-wound healing efficacy.ZIP

Le Li (356426)
Bingjie Yu (694763)
Yingji Lai (17902871)
Siyuan Shen (649230)
Yawei Yan (8399274)
Guojun Dong (389687)
Xiangyun Gao (400310)
Yanrong Cao (10164943)
Caojie Ge (17902874)
Liqin Zhu (8982803)
Huan Liu (281351)
Shanhui Tao (17902877)
Zhiang Yao (17902880)
Shijun Li (165293)
Xiaojie Wang (166442)
Qi Hui (783264)

February 2024

Introduction: Human basic fibroblast growth factor (hbFGF) is a highly valuable multifunctional prot...

Cloning, Expression and Functional Characterization of In-House Prepared Human Basic Fibroblast Growth Factor

Hassan Rassouli
Mohammad Sharif Tabe Bordbar
Mehran Rezaei Larijani
Mohammad Pakzad
Hossein Baharvand
Ghasem Hosseini Salekdeh

January 2013

Objective: Human basic fibroblast growth factor (bFGF) plays an important role in cellular prolifera...

MOESM6 of Production of a functional cell wall-anchored minicellulosome by recombinant Clostridium acetobutylicum ATCC 824

Benjamin Willson (3448415)
Katalin Kovรกcs (3448421)
Tom Wilding-Steele (3448418)
Robert Markus (635222)
Klaus Winzer (50786)
Nigel Minton (565961)

May 2016

Additional file 6. Sequences of the proteins and genetic components used in the study. This file con...

MOESM5 of A particular silent codon exchange in a recombinant gene greatly influences host cell metabolic activity

Natalie Rahmen (3496205)
Christian Schlupp (3496196)
Hitoshi Mitsunaga (3496214)
Alexander Fulton (2579620)
Tita Aryani (3496211)
Lara Esch (3496199)
Ulrich Schaffrath (3496208)
Eiichiro Fukuzaki (3496202)
Karl-Erich Jaeger (125594)
Jochen Büchs (3437060)

October 2015

Additional file 5. Product formation and sum of relative transcript abundances of four E. coli BL21(...

Preparation of cFGF-21.

Pengfei Xu (301785)
Yingjie Zhang (1234773)
Xinghao Jiang (2802682)
Junyan Li (238758)
Liying Song (2802685)
Mir Hasson Khoso (2802694)
Yunye Liu (2802691)
Qiang Wu (31071)
Guiping Ren (413780)
Deshan Li (2802688)

May 2016

(A) SDS-PAGE gel analysis of cFGF-21 expression. Lane 1: Protein molecular mass marker; lane 2: T...

Human FGF-21 is digested by FAP but not PREP.

Andrew L. Coppage (2560108)
Kathryn R. Heard (2560111)
Matthew T. DiMare (2560105)
Yuxin Liu (505575)
Wengen Wu (1883827)
Jack H. Lai (1883839)
William W. Bachovchin (1883854)

March 2016

(A) Human FGF-21 is cleaved by FAP. Recombinant human FGF-21 was digested by recombinant human FA...

MOESM1 of High-efficiency expression and secretion of human FGF21 in Bacillus subtilis by intercalation of a mini-cistron cassette and combinatorial optimization of cell regulatory components

Dandan Li (391591)
Gang Fu (728533)
Ran Tu (4349167)
Zhaoxia Jin (1500538)
Dawei Zhang (58801)

February 2019

Additional file 1: Figure S1. The relative expression level of rhFGF21 from different B. subtilis st...

MOESM4 of High-efficiency expression and secretion of human FGF21 in Bacillus subtilis by intercalation of a mini-cistron cassette and combinatorial optimization of cell regulatory components

Dandan Li (391591)
Gang Fu (728533)
Ran Tu (4349167)
Zhaoxia Jin (1500538)
Dawei Zhang (58801)

February 2019

Additional file 4: Figure S4. ERK1/2 phosphorylation assay of hFGF21 standard and rhFGF21 from Kno6c...

MOESM3 of High-efficiency expression and secretion of human FGF21 in Bacillus subtilis by intercalation of a mini-cistron cassette and combinatorial optimization of cell regulatory components

Dandan Li (391591)
Gang Fu (728533)
Ran Tu (4349167)
Zhaoxia Jin (1500538)
Dawei Zhang (58801)

February 2019

Additional file 3: Figure S3. LCâMS analysis of rhFGF21 protein expressed and purified from B. sub...

MOESM2 of High-efficiency expression and secretion of human FGF21 in Bacillus subtilis by intercalation of a mini-cistron cassette and combinatorial optimization of cell regulatory components

Dandan Li (391591)
Gang Fu (728533)
Ran Tu (4349167)
Zhaoxia Jin (1500538)
Dawei Zhang (58801)

February 2019

Additional file 2: Figure S2. SDS-PAGE analysis of the purified secreted rhFGF21 with C-terminal His...

High-efficiency expression and secretion of human FGF21 in Bacillus subtilis by intercalation of a mini-cistron cassette and combinatorial optimization of cell regulatory components

Dandan Li
Gang Fu
Ran Tu
Zhaoxia Jin
Dawei Zhang

January 2019

Abstract Background Recombinant human Fibroblast growth factor 21 (rhFGF21) is an endocrine hormone ...

Cloning, expression and purification of human fibroblast growth factor 21 in Escherichia coli and Pichia pastoris / Farnaz Emamdoust

Farnaz , Emamdoust

May 2018

Fibroblast Growth Factor 21 (FGF21) is a novel target with potential anti diabetic properties that ...

MOESM1 of Combinatorial pathway enzyme engineering and host engineering overcomes pyruvate overflow and enhances overproduction of N-acetylglucosamine in Bacillus subtilis

Wenlong Ma (6175187)
Yanfeng Liu (405866)
Xueqin Lv (279878)
Jianghua Li (390525)
Guocheng Du (390526)
Long Liu (390523)

January 2019

Additional file 1: Table S1. Primers used in this study. Fig S1. The mutagenesis selection process. ...

In vitro activity of Fc-FGF21(RG) relative to native FGF21 and Fc-FGF21 wild type.

Randy Hecht (117873)
Yue-Sheng Li (117877)
Jeonghoon Sun (117881)
Ed Belouski (117884)
Michael Hall (117887)
Todd Hager (117889)
Junming Yie (117890)
Wei Wang (17594)
Dwight Winters (117892)
Stephen Smith (117894)
Chris Spahr (117896)
Lei-Ting Tam (117897)
Zhongnan Shen (117899)
Shanaka Stanislaus (117901)
Narumol Chinookoswong (117903)
Yvonne Lau (117907)
Allen Sickmier (117911)
Mark Leo Michaels (117917)
Thomas Boone (117922)
Murielle M. Véniant (117927)
Jing Xu (15337)

November 2012

(A) Structural diagram of Fc-FGF21(RG), an E coli-expressed homodimeric Fc-FGF21 fusion protein c...

Table1_Scaling up production of recombinant human basic fibroblast growth factor in an Escherichia coli BL21(DE3) plysS strain and evaluation of its pro-wound healing efficacy.DOCX

Le Li (356426)
Bingjie Yu (694763)
Yingji Lai (17902871)
Siyuan Shen (649230)
Yawei Yan (8399274)
Guojun Dong (389687)
Xiangyun Gao (400310)
Yanrong Cao (10164943)
Caojie Ge (17902874)
Liqin Zhu (8982803)
Huan Liu (281351)
Shanhui Tao (17902877)
Zhiang Yao (17902880)
Shijun Li (165293)
Xiaojie Wang (166442)
Qi Hui (783264)

February 2024

Introduction: Human basic fibroblast growth factor (hbFGF) is a highly valuable multifunctional prot...

DataSheet5_Scaling up production of recombinant human basic fibroblast growth factor in an Escherichia coli BL21(DE3) plysS strain and evaluation of its pro-wound healing efficacy.ZIP

Le Li (356426)
Bingjie Yu (694763)
Yingji Lai (17902871)
Siyuan Shen (649230)
Yawei Yan (8399274)
Guojun Dong (389687)
Xiangyun Gao (400310)
Yanrong Cao (10164943)
Caojie Ge (17902874)
Liqin Zhu (8982803)
Huan Liu (281351)
Shanhui Tao (17902877)
Zhiang Yao (17902880)
Shijun Li (165293)
Xiaojie Wang (166442)
Qi Hui (783264)

February 2024

Introduction: Human basic fibroblast growth factor (hbFGF) is a highly valuable multifunctional prot...

Cloning, Expression and Functional Characterization of In-House Prepared Human Basic Fibroblast Growth Factor

Hassan Rassouli
Mohammad Sharif Tabe Bordbar
Mehran Rezaei Larijani
Mohammad Pakzad
Hossein Baharvand
Ghasem Hosseini Salekdeh

January 2013

Objective: Human basic fibroblast growth factor (bFGF) plays an important role in cellular prolifera...

MOESM6 of Production of a functional cell wall-anchored minicellulosome by recombinant Clostridium acetobutylicum ATCC 824

Benjamin Willson (3448415)
Katalin Kovรกcs (3448421)
Tom Wilding-Steele (3448418)
Robert Markus (635222)
Klaus Winzer (50786)
Nigel Minton (565961)

May 2016

Additional file 6. Sequences of the proteins and genetic components used in the study. This file con...

MOESM5 of A particular silent codon exchange in a recombinant gene greatly influences host cell metabolic activity

Natalie Rahmen (3496205)
Christian Schlupp (3496196)
Hitoshi Mitsunaga (3496214)
Alexander Fulton (2579620)
Tita Aryani (3496211)
Lara Esch (3496199)
Ulrich Schaffrath (3496208)
Eiichiro Fukuzaki (3496202)
Karl-Erich Jaeger (125594)
Jochen Büchs (3437060)

October 2015

Additional file 5. Product formation and sum of relative transcript abundances of four E. coli BL21(...

Preparation of cFGF-21.

Pengfei Xu (301785)
Yingjie Zhang (1234773)
Xinghao Jiang (2802682)
Junyan Li (238758)
Liying Song (2802685)
Mir Hasson Khoso (2802694)
Yunye Liu (2802691)
Qiang Wu (31071)
Guiping Ren (413780)
Deshan Li (2802688)

May 2016

(A) SDS-PAGE gel analysis of cFGF-21 expression. Lane 1: Protein molecular mass marker; lane 2: T...

Human FGF-21 is digested by FAP but not PREP.

Andrew L. Coppage (2560108)
Kathryn R. Heard (2560111)
Matthew T. DiMare (2560105)
Yuxin Liu (505575)
Wengen Wu (1883827)
Jack H. Lai (1883839)
William W. Bachovchin (1883854)

March 2016

(A) Human FGF-21 is cleaved by FAP. Recombinant human FGF-21 was digested by recombinant human FA...

MOESM5 of High-efficiency expression and secretion of human FGF21 in Bacillus subtilis by intercalation of a mini-cistron cassette and combinatorial optimization of cell regulatory components

Abstract

Extracted data

MOESM5 of High-efficiency expression and secretion of human FGF21 in Bacillus subtilis by intercalation of a mini-cistron cassette and combinatorial optimization of cell regulatory components

Abstract

Extracted data

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