MOESM2 of High-efficiency expression and secretion of human FGF21 in Bacillus subtilis by intercalation of a mini-cistron cassette and combinatorial optimization of cell regulatory components

Additional file 2: Figure S2. SDS-PAGE analysis of the purified secreted rhFGF21 with C-terminal His-tag from B. subtilis. Lane 1 represents the protein marker. Lane 2–4 represents different load volume of purified rhFGF21 from B. subtilis. Lane 5 (std) represents the rhFGF21 standard sample as a control. Arrow indicates the band of rhFGF21 protein