Additional file 4: Figure S4. ERK1/2 phosphorylation assay of hFGF21 standard and rhFGF21 from Kno6cf in HEK-hBKL cells with stable over-expression of βKlotho (Klb)
Additional file 1: Table S1. Primers for expression plasmid construction with different signal pepti...
Additional file 1: Figure S1. Subcellular fractionation of cells expressing ssTorA/hEGF
An efficient expression/secretion vector, designated pM2Veg, was constructed for extracellular produ...
Additional file 5: Table S1. Summary of in vitro activities of rhFGF21 from Kno6cf and hFGF21 standa...
Additional file 1: Figure S1. The relative expression level of rhFGF21 from different B. subtilis st...
Additional file 3: Figure S3. LCâMS analysis of rhFGF21 protein expressed and purified from B. sub...
Additional file 2: Figure S2. SDS-PAGE analysis of the purified secreted rhFGF21 with C-terminal His...
Abstract Background Recombinant human Fibroblast growth factor 21 (rhFGF21) is an endocrine hormone ...
Additional file 1: Figure S1. FHT production by HMS174(DE3) during the characterization-cultivation ...
Additional file 1: Figure S1. N-terminal amino acid sequence results of inclusion bodies. (A) Standa...
<p>(A) Structural diagram of Fc-FGF21(RG), an E coli-expressed homodimeric Fc-FGF21 fusion protein c...
Additional file 1: Table S1. Primers used in this study. Fig S1. The mutagenesis selection process. ...
Additional file 2: Fig. S1. Figure S1. Cellular localization of FHF proteins. A Western blotting ana...
Figure S1. (A) eIF4E and SmBIT-eIF4E (indicated with black arrows), eIF4G or eIF4G604–646-LgBiT leve...
Additional file 2: Figure S2. SMYD5 and H4K20me3 co-occupancy in ES cells. (A) Venn diagram showing ...
Additional file 1: Table S1. Primers for expression plasmid construction with different signal pepti...
Additional file 1: Figure S1. Subcellular fractionation of cells expressing ssTorA/hEGF
An efficient expression/secretion vector, designated pM2Veg, was constructed for extracellular produ...
Additional file 5: Table S1. Summary of in vitro activities of rhFGF21 from Kno6cf and hFGF21 standa...
Additional file 1: Figure S1. The relative expression level of rhFGF21 from different B. subtilis st...
Additional file 3: Figure S3. LCâMS analysis of rhFGF21 protein expressed and purified from B. sub...
Additional file 2: Figure S2. SDS-PAGE analysis of the purified secreted rhFGF21 with C-terminal His...
Abstract Background Recombinant human Fibroblast growth factor 21 (rhFGF21) is an endocrine hormone ...
Additional file 1: Figure S1. FHT production by HMS174(DE3) during the characterization-cultivation ...
Additional file 1: Figure S1. N-terminal amino acid sequence results of inclusion bodies. (A) Standa...
<p>(A) Structural diagram of Fc-FGF21(RG), an E coli-expressed homodimeric Fc-FGF21 fusion protein c...
Additional file 1: Table S1. Primers used in this study. Fig S1. The mutagenesis selection process. ...
Additional file 2: Fig. S1. Figure S1. Cellular localization of FHF proteins. A Western blotting ana...
Figure S1. (A) eIF4E and SmBIT-eIF4E (indicated with black arrows), eIF4G or eIF4G604–646-LgBiT leve...
Additional file 2: Figure S2. SMYD5 and H4K20me3 co-occupancy in ES cells. (A) Venn diagram showing ...
Additional file 1: Table S1. Primers for expression plasmid construction with different signal pepti...
Additional file 1: Figure S1. Subcellular fractionation of cells expressing ssTorA/hEGF
An efficient expression/secretion vector, designated pM2Veg, was constructed for extracellular produ...