Additional file 3 of Optimization of expression, purification and secretion of functional recombinant human growth hormone in Escherichia coli using modified staphylococcal protein a signal peptide

Expression System for Recombinant Human Growth Hormone Production from Bacillus subtilis

ÖZDAMAR, HASAN TUNÇER
Sentuerk, Birguel
Yilmaz, Oezge Deniz
Calik, Guezide
Celik, Eda
Çalık, Pınar

January 2009

We demonstrate for the first time, an expression system mimicking serine alkaline protease synthesis...

Expression and purification of human epidermal growth factor (hEGF) fused with GB1

Xueming Zheng
Xin Wu
Xingli Fu
Dongping Dai
Feihu Wang

July 2016

Fusion expression is a promising strategy for the production of bioactive peptides in Escherichia co...

Expression system for synthesis and purification of recombinant human growth hormone in Pichia pastoris and structural analysis by MALDI-ToF mass spectrometry

Çalık, Pınar
Orman, Mehmet Ali
Celik, Eda
Halloran, S. Mitchell
Calik, Guezide
Ozdamar, Tuncer H.

January 2008

An expression system in Pichia pastoris for the production and purification of recombinant human gro...

Additional file 2 of Optimization of expression, purification and secretion of functional recombinant human growth hormone in Escherichia coli using modified staphylococcal protein a signal peptide

Garshasb Rigi (11283161)
Amin Rostami (810255)
Habib Ghomi (11283164)
Gholamreza Ahmadian (3410240)
Vasiqe Sadat Mirbagheri (11283167)
Meisam Jeiranikhameneh (3803341)
Majid Vahed (5003837)
Sahel Rahimi (11283170)

August 2021

Additional file 2. The original, unprocessed and uncropped version of the Fig. 7 in manuscript

Additional file 4 of Optimization of expression, purification and secretion of functional recombinant human growth hormone in Escherichia coli using modified staphylococcal protein a signal peptide

Garshasb Rigi (11283161)
Amin Rostami (810255)
Habib Ghomi (11283164)
Gholamreza Ahmadian (3410240)
Vasiqe Sadat Mirbagheri (11283167)
Meisam Jeiranikhameneh (3803341)
Majid Vahed (5003837)
Sahel Rahimi (11283170)

August 2021

Additional file 4. The original, unprocessed and uncropped version of the Fig. 9 in manuscript

Additional file 1 of Optimization of expression, purification and secretion of functional recombinant human growth hormone in Escherichia coli using modified staphylococcal protein a signal peptide

Garshasb Rigi (11283161)
Amin Rostami (810255)
Habib Ghomi (11283164)
Gholamreza Ahmadian (3410240)
Vasiqe Sadat Mirbagheri (11283167)
Meisam Jeiranikhameneh (3803341)
Majid Vahed (5003837)
Sahel Rahimi (11283170)

August 2021

Additional file 1. The schematic view of the pET26 plasmid as well as the designed gene structure

Additional file 5 of Optimization of expression, purification and secretion of functional recombinant human growth hormone in Escherichia coli using modified staphylococcal protein a signal peptide

Garshasb Rigi (11283161)
Amin Rostami (810255)
Habib Ghomi (11283164)
Gholamreza Ahmadian (3410240)
Vasiqe Sadat Mirbagheri (11283167)
Meisam Jeiranikhameneh (3803341)
Majid Vahed (5003837)
Sahel Rahimi (11283170)

August 2021

Additional file 5. After generating the standard curves for each test by plotting the absorbance ver...

Expression, secretion and folding of human growth hormone in Escherichia coli Purification and characterization

Becker, Gerald W.
Hsiung, Hansen M.

August 1986

AbstractAn efficient secretion vector containing a gene coding for an E. coli signal peptide fused t...

CONSTRUCTION OF RECOMBINANT PLASMIDS FOR PERIPLASMIC EXPRESSION OF HUMAN GROWTH HORMONE IN ESCHERICHIA COLI UNDER T7 AND LAC PROMOTERS

J. Kiany
M. Ahmadzadeh Raji
M. H. Sanati

September 2016

In order to study the periplasmic expression of human growth hormone (hGH) in Escherichia coli, the ...

High level expression of recombinant human growth hormone in Escherichia coli: crucial role of translation initiation region

Mahsa Ghavim
Khalil Abnous
Fatemeh Arasteh
Sahar Taghavi
Maryam Sadat Nabavinia
Mona Alibolandi
Mohammad Ramezani

January 2017

For high-throughput production of recombinant protein in Escherichia coli (E. coli ), besides import...

Optimizing expression of recombinant porcine growth hormone in E. coli / by Carol Senn.

Senn, Carol

January 1995

Bibliography: leaves 239-259.259 leaves : ill. ; 30 cm.Concentrations of minimal medium components a...

MOESM1 of Novel signal peptides improve the secretion of recombinant Staphylococcus aureus Alpha toxinH35L in Escherichia coli

SooJin Han (4021826)
Shushil Machhi (2608651)
Mark Berge (3597410)
Guoling Xi (4021835)
Thomas Linke (4021832)
Ronald Schoner (4021829)

May 2017

Additional file 1: Table S1. Identified peptide sequences of secreted ATH35L in culture medium. Figu...

Purification and Biochemical Characterization of Recombinant Human Placental Growth Hormone Produced in Escherichia Coli

Igout, Ahmed
Van Beeumen, Jozef
Frankenne, Francis
Scippo, Marie-Louise
DEVREESE, Bart
Hennen, Georges

January 1993

The hGH-V (or hGH-2) gene codes for human placental growth hormone (hPGH). Secretion of hPGH is cont...

Human growth hormone: complementary DNA cloning and expression in bacteria

Martial, Joseph
Hallewell, R. A.
Baxter, J. D.
Goodman, H. M.

January 1979

The nucleotide sequence of a DNA complementary to human growth hormone messenger RNA was cloned; it ...

Endogenous signal peptides in recombinant protein production by Pichia pastoris: From in-silico analysis to fermentation

Massahi, Aslan
Çalık, Pınar

November 2016

For extracellular recombinant protein production, the efficiency of five endogenous secretion signal...

Expression System for Recombinant Human Growth Hormone Production from Bacillus subtilis

ÖZDAMAR, HASAN TUNÇER
Sentuerk, Birguel
Yilmaz, Oezge Deniz
Calik, Guezide
Celik, Eda
Çalık, Pınar

January 2009

We demonstrate for the first time, an expression system mimicking serine alkaline protease synthesis...

Expression and purification of human epidermal growth factor (hEGF) fused with GB1

Xueming Zheng
Xin Wu
Xingli Fu
Dongping Dai
Feihu Wang

July 2016

Fusion expression is a promising strategy for the production of bioactive peptides in Escherichia co...

Expression system for synthesis and purification of recombinant human growth hormone in Pichia pastoris and structural analysis by MALDI-ToF mass spectrometry

Çalık, Pınar
Orman, Mehmet Ali
Celik, Eda
Halloran, S. Mitchell
Calik, Guezide
Ozdamar, Tuncer H.

January 2008

An expression system in Pichia pastoris for the production and purification of recombinant human gro...

Additional file 2 of Optimization of expression, purification and secretion of functional recombinant human growth hormone in Escherichia coli using modified staphylococcal protein a signal peptide

Garshasb Rigi (11283161)
Amin Rostami (810255)
Habib Ghomi (11283164)
Gholamreza Ahmadian (3410240)
Vasiqe Sadat Mirbagheri (11283167)
Meisam Jeiranikhameneh (3803341)
Majid Vahed (5003837)
Sahel Rahimi (11283170)

August 2021

Additional file 2. The original, unprocessed and uncropped version of the Fig. 7 in manuscript

Additional file 4 of Optimization of expression, purification and secretion of functional recombinant human growth hormone in Escherichia coli using modified staphylococcal protein a signal peptide

Garshasb Rigi (11283161)
Amin Rostami (810255)
Habib Ghomi (11283164)
Gholamreza Ahmadian (3410240)
Vasiqe Sadat Mirbagheri (11283167)
Meisam Jeiranikhameneh (3803341)
Majid Vahed (5003837)
Sahel Rahimi (11283170)

August 2021

Additional file 4. The original, unprocessed and uncropped version of the Fig. 9 in manuscript

Additional file 1 of Optimization of expression, purification and secretion of functional recombinant human growth hormone in Escherichia coli using modified staphylococcal protein a signal peptide

Garshasb Rigi (11283161)
Amin Rostami (810255)
Habib Ghomi (11283164)
Gholamreza Ahmadian (3410240)
Vasiqe Sadat Mirbagheri (11283167)
Meisam Jeiranikhameneh (3803341)
Majid Vahed (5003837)
Sahel Rahimi (11283170)

August 2021

Additional file 1. The schematic view of the pET26 plasmid as well as the designed gene structure

Additional file 5 of Optimization of expression, purification and secretion of functional recombinant human growth hormone in Escherichia coli using modified staphylococcal protein a signal peptide

Garshasb Rigi (11283161)
Amin Rostami (810255)
Habib Ghomi (11283164)
Gholamreza Ahmadian (3410240)
Vasiqe Sadat Mirbagheri (11283167)
Meisam Jeiranikhameneh (3803341)
Majid Vahed (5003837)
Sahel Rahimi (11283170)

August 2021

Additional file 5. After generating the standard curves for each test by plotting the absorbance ver...

Expression, secretion and folding of human growth hormone in Escherichia coli Purification and characterization

Becker, Gerald W.
Hsiung, Hansen M.

August 1986

AbstractAn efficient secretion vector containing a gene coding for an E. coli signal peptide fused t...

CONSTRUCTION OF RECOMBINANT PLASMIDS FOR PERIPLASMIC EXPRESSION OF HUMAN GROWTH HORMONE IN ESCHERICHIA COLI UNDER T7 AND LAC PROMOTERS

J. Kiany
M. Ahmadzadeh Raji
M. H. Sanati

September 2016

In order to study the periplasmic expression of human growth hormone (hGH) in Escherichia coli, the ...

High level expression of recombinant human growth hormone in Escherichia coli: crucial role of translation initiation region

Mahsa Ghavim
Khalil Abnous
Fatemeh Arasteh
Sahar Taghavi
Maryam Sadat Nabavinia
Mona Alibolandi
Mohammad Ramezani

January 2017

For high-throughput production of recombinant protein in Escherichia coli (E. coli ), besides import...

Optimizing expression of recombinant porcine growth hormone in E. coli / by Carol Senn.

Senn, Carol

January 1995

Bibliography: leaves 239-259.259 leaves : ill. ; 30 cm.Concentrations of minimal medium components a...

MOESM1 of Novel signal peptides improve the secretion of recombinant Staphylococcus aureus Alpha toxinH35L in Escherichia coli

SooJin Han (4021826)
Shushil Machhi (2608651)
Mark Berge (3597410)
Guoling Xi (4021835)
Thomas Linke (4021832)
Ronald Schoner (4021829)

May 2017

Additional file 1: Table S1. Identified peptide sequences of secreted ATH35L in culture medium. Figu...

Purification and Biochemical Characterization of Recombinant Human Placental Growth Hormone Produced in Escherichia Coli

Igout, Ahmed
Van Beeumen, Jozef
Frankenne, Francis
Scippo, Marie-Louise
DEVREESE, Bart
Hennen, Georges

January 1993

The hGH-V (or hGH-2) gene codes for human placental growth hormone (hPGH). Secretion of hPGH is cont...

Human growth hormone: complementary DNA cloning and expression in bacteria

Martial, Joseph
Hallewell, R. A.
Baxter, J. D.
Goodman, H. M.

January 1979

The nucleotide sequence of a DNA complementary to human growth hormone messenger RNA was cloned; it ...

Endogenous signal peptides in recombinant protein production by Pichia pastoris: From in-silico analysis to fermentation

Massahi, Aslan
Çalık, Pınar

November 2016

For extracellular recombinant protein production, the efficiency of five endogenous secretion signal...

Expression System for Recombinant Human Growth Hormone Production from Bacillus subtilis

ÖZDAMAR, HASAN TUNÇER
Sentuerk, Birguel
Yilmaz, Oezge Deniz
Calik, Guezide
Celik, Eda
Çalık, Pınar

January 2009

We demonstrate for the first time, an expression system mimicking serine alkaline protease synthesis...

Expression and purification of human epidermal growth factor (hEGF) fused with GB1

Xueming Zheng
Xin Wu
Xingli Fu
Dongping Dai
Feihu Wang

July 2016

Fusion expression is a promising strategy for the production of bioactive peptides in Escherichia co...

Expression system for synthesis and purification of recombinant human growth hormone in Pichia pastoris and structural analysis by MALDI-ToF mass spectrometry

Çalık, Pınar
Orman, Mehmet Ali
Celik, Eda
Halloran, S. Mitchell
Calik, Guezide
Ozdamar, Tuncer H.

January 2008

An expression system in Pichia pastoris for the production and purification of recombinant human gro...

Additional file 3 of Optimization of expression, purification and secretion of functional recombinant human growth hormone in Escherichia coli using modified staphylococcal protein a signal peptide

Abstract

Extracted data

Additional file 3 of Optimization of expression, purification and secretion of functional recombinant human growth hormone in Escherichia coli using modified staphylococcal protein a signal peptide

Abstract

Extracted data

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