The effect of MS-275 on histone acetylation in mouse Kera-308 cells.

(A) Representative Western blots depicting dose dependent increases in histone H3K9 acetylation upon treatment with the indicated amount of drug for 24 h. H3K9ac and total H3 blots were run separately on different gels using aliquots from the same sample preparations and processed in parallel. Uncropped Western blots are available as S5A Fig. (B) H3K9 acetylation levels were normalized to total H3 and quantified by densitometry using ImageJ (n = 3, unpaired t test, *p p < 0.001).