VPA treatment increases H3K9 acetylation without affecting DNA methylation of donor cells.

<p>(A) Relative average intensity of H3K9 acetylation (H3K9ac) of control and VPA-treated cells. (B) Relative average intensity of 5-methylcytosine (5-mC) of control and VPA-treated cells. (C–D) Immunofluorescence labeling for H3K9ac of control (C) and VPA-treated cells (D). (E–F) Immunofluorescence labeling for 5-mC of control (E) and VPA-treated cells (F). The (*) denotes a significant difference between experimental groups (P<0.0001). All images were taken in the same magnification (200×).